Fawzy Eissa I, El Makawy Aida I, El-Bamby M Mahmoud, Elhamalawy H Osama
Department of Environment and Bio-Agriculture, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Department of Cell Biology, National Research Center, Dokki, Giza, Egypt.
Toxicol Rep. 2018 Aug 17;5:857-863. doi: 10.1016/j.toxrep.2018.08.014. eCollection 2018.
The present study was conducted to evaluate the ameliorative role of pumpkin seed oil (PSO) against potential adverse effects of bisphenol-A (BPA) in male mice. BPA was administered to the mice orally at a dose of 50 mg/kg body weight once a day for 28 successive days. While, PSO was administered to the mice orally at 1 mL/kg b w either before, with or after treatment of BPA, once a day for 28 successive days. The studied parameters were DNA damage evaluation using comet assay in liver and testes cells and micronucleus test in bone marrow; and histopathological examination of liver and testes tissues. Results revealed that BPA induced DNA damage in tested cells and marked histopathological alterations in liver and testes. In contrast, PSO treatments alleviated DNA damage and improved the histopathological alterations in liver and testes tissues. Furthermore, administration of mice with the PSO before BPA treatment was the best regimen in the alleviation of the adverse effects of BPA, followed by administration of PSO after then with treatment of BPA. It can be concluded that PSO may has a protective role against BPA genotoxicity and histopathological alterations in male mice.
本研究旨在评估南瓜籽油（PSO）对双酚A（BPA）在雄性小鼠中潜在不良影响的改善作用。以50毫克/千克体重的剂量每天一次给小鼠口服BPA，连续28天。同时，在BPA治疗前、治疗期间或治疗后，以1毫升/千克体重的剂量每天一次给小鼠口服PSO，连续28天。研究参数包括使用彗星试验评估肝脏和睾丸细胞中的DNA损伤以及骨髓中的微核试验；以及肝脏和睾丸组织的组织病理学检查。结果显示，BPA诱导了受试细胞中的DNA损伤以及肝脏和睾丸中明显的组织病理学改变。相比之下，PSO处理减轻了DNA损伤，并改善了肝脏和睾丸组织中的组织病理学改变。此外，在BPA治疗前给小鼠服用PSO是减轻BPA不良影响的最佳方案，其次是在BPA治疗后服用PSO。可以得出结论，PSO可能对雄性小鼠的BPA遗传毒性和组织病理学改变具有保护作用。
