Lee Jun Beom, Byeon Jin Hee, Jang Ho Am, Kim Jiyeun Kate, Yoo Jin Wook, Kikuchi Yoshitomo, Lee Bok Luel
Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
Department of Microbiology, Kosin University College of Medicine, Busan 602-703, South Korea.
FEBS Lett. 2015 Sep 14;589(19 Pt B):2784-90. doi: 10.1016/j.febslet.2015.08.022. Epub 2015 Aug 28.
We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-l-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile ΔamiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, ΔamiC mutant cells were unable to establish symbiotic association. But, ΔamiC mutant complemented with amiC gene restored in vivo symbiotic association. ΔamiC mutant cultured in minimal medium restored their motility with single-celled morphology. When ΔamiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont.
我们构建了一种伯克霍尔德氏菌突变体，该突变体缺乏参与肽聚糖降解的N - 乙酰胞壁酰 - L - 丙氨酸酰胺酶AmiC。当将携带链状形态的无运动能力的ΔamiC突变体伯克霍尔德氏菌细胞经口给予稻绿蝽昆虫时，ΔamiC突变体细胞无法建立共生关系。但是，用amiC基因互补的ΔamiC突变体恢复了体内共生关系。在基本培养基中培养的ΔamiC突变体恢复了单细胞形态的运动能力。当将具有单细胞形态的ΔamiC突变体细胞给予宿主昆虫时，该突变体建立了正常的共生关系，这表明细菌的运动能力对于宿主昆虫与伯克霍尔德氏菌共生体之间的成功共生至关重要。
