Arciniega-Martínez Ivonne Maciel, Campos-Rodríguez Rafael, Drago-Serrano María Elisa, Sánchez-Torres Luvia Enid, Cruz-Hernández Teresita Rocío, Reséndiz-Albor Aldo Arturo
Research and Graduate Studies Section, Superior School of Medicine, National Polytechnic Institute, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomás, CP 11340, Mexico, DF, Mexico.
Department of Biological Systems, Autonomous Metropolitan University Campus Xochimilco, Calzada del Hueso No 1100, Col. Villa Quietud, CP 04960, Mexico, DF, Mexico.
Arch Immunol Ther Exp (Warsz). 2016 Feb;64(1):57-63. doi: 10.1007/s00005-015-0358-6. Epub 2015 Aug 30.
Bovine lactoferrin (bLf) up-modulates intestinal IgA that is essential for homeostasis and which might confer protection to the distal small intestine that is vulnerable to inflammation. This study analyzed the effects of bLf administered orally on the IgA response at inductive (Peyer's patches) and effector (lamina propria) sites of the distal small intestine in mice. Groups of five healthy male BALB/c mice were orally treated with 5 mg of bLf for 7, 14, 21, or 28 days. Then, mice were killed and the distal small intestine was dissected. Intestinal fluid samples were analyzed to determine IgA and IgM levels by enzyme-immuno assay. Peyer's patches and lamina propria were analyzed for IgA(+) or IgM(+) plasma cells, B, CD4(+) T and CD8(+) T cells as well as CD4(+) T cells positive for either pro-inflammatory cytokines [tumor necrosis factor (TNF)-α, interferon-γ and interleukin (IL)-12] or for IgA-producing ILs (IL-4, -5, -10 and -6) by cytofluorometry. Antibodies, antibody-secreting cells, and B and T responses in both Peyer's patches and lamina propria were higher in bLf-treated than bLf-untreated mice. The generation of IL-10 and IL-6 CD4(+) T cells in Peyer's patches or TNF-α and IL-12 CD4(+) T cells in lamina propria showed similar response patterns. On days 14 and 28, cytokine/IL CD4(+) T cell responses were increased in Peyer's patches or decreased in lamina propria. The effect of bLf on the elicitation of IgA indicates a potential application of bLf as a nutraceutical to control inflammation in the distal small intestine.
牛乳铁蛋白（bLf）上调肠道IgA，这对体内平衡至关重要，并且可能为易受炎症影响的远端小肠提供保护。本研究分析了口服bLf对小鼠远端小肠诱导部位（派尔集合淋巴结）和效应部位（固有层）IgA反应的影响。将五组健康雄性BALB/c小鼠分别用5毫克bLf口服处理7天、14天、21天或28天。然后，处死小鼠并解剖远端小肠。通过酶免疫测定分析肠液样本以确定IgA和IgM水平。通过细胞荧光测定法分析派尔集合淋巴结和固有层中的IgA(+)或IgM(+)浆细胞、B细胞、CD4(+) T细胞和CD8(+) T细胞，以及促炎细胞因子[肿瘤坏死因子（TNF）-α、干扰素-γ和白细胞介素（IL）-12]或产生IgA的白细胞介素（IL-4、-5、-10和-6）呈阳性的CD4(+) T细胞。与未用bLf处理的小鼠相比，用bLf处理的小鼠的派尔集合淋巴结和固有层中的抗体、抗体分泌细胞以及B细胞和T细胞反应更高。派尔集合淋巴结中IL-10和IL-6 CD4(+) T细胞的产生或固有层中TNF-α和IL-12 CD4(+) T细胞的产生表现出相似的反应模式。在第14天和第28天，派尔集合淋巴结中的细胞因子/白细胞介素CD4(+) T细胞反应增加，而固有层中的反应则减少。bLf对IgA诱导的作用表明bLf作为一种营养保健品在控制远端小肠炎症方面具有潜在应用。
