Laboratory of Epidemiology and Artificial Intelligence, Faculty of Public and One Health, School of Health Sciences, University of Thessaly, Karditsa, Greece.
UQ Centre for Clinical Research, Faculty of Medicine, the University of Queensland, Herston, Queensland, Australia.
Pathog Glob Health. 2023 May;117(3):259-272. doi: 10.1080/20477724.2022.2097830. Epub 2022 Jul 11.
infection is one of the most frequently reported foodborne diseases with approximately 230,000 and 1.5 million cases each year in Europe and the USA, respectively. Culture methods are the reference for the diagnosis of infections; however, these methods are complex and time-consuming. Multiplex nucleic acid amplification test is favored due to its rapidity, automatization in the procedure followed and the quick simultaneous testing of numerous foodborne pathogens. The aim of this meta-analysis was to evaluate the accuracy of these tests for the diagnosis of infection. Scopus, Science Direct, PubMed, Web of Science, and Mendeley were searched for peer-reviewed articles. The split component synthesis method with the use of the inverse variance heterogeneity model was chosen for the quantitative meta-analysis. Sensitivity analysis was performed by age category and index test. The literature search found 34 studies involving 28,105 patients with suspected gastroenteritis. The sensitivity and specificity were 95.3% (92.3; 97.1) and 97.1% (95.1; 98.3), respectively, and AUC (area under the curve) was 0.963 (0.947; 0.974). Pediatric patients had a lower sensitivity (87.4, 48.2; 98.1) and higher specificity (99.2, 91.6; 99.9) estimate compared to all ages category (sensitivity 95.3, 91.3; 97.5, specificity 96.7, 93.7; 98.3). Among the various index tests, Seeplex/Allplex and Amplidiag/Novodiag had the lowest estimate for sensitivity (88.9, 73.8; 95.8) and specificity (95.2, 86; 98.4), respectively. BDMax had the highest (sensitivity 98.1, 96.1; 99 and specificity 98.5, 97; 99.3). Multiplex nucleic acid tests showed excellent accuracy and could play an influential role in diagnosing infections.
感染是最常报告的食源性疾病之一，在欧洲和美国，每年分别约有 23 万和 150 万例病例。培养方法是感染诊断的参考方法；然而，这些方法复杂且耗时。由于其快速性、程序自动化以及对众多食源性病原体的快速同步检测，多重核酸扩增检测受到青睐。本荟萃分析旨在评估这些检测方法对感染诊断的准确性。使用 Scopus、Science Direct、PubMed、Web of Science 和 Mendeley 搜索同行评议文章。选择使用逆方差异质性模型的拆分成分综合方法进行定量荟萃分析。通过年龄类别和指标测试进行敏感性分析。文献检索发现 34 项研究涉及 28105 例疑似胃肠炎患者。敏感性和特异性分别为 95.3%（92.3；97.1）和 97.1%（95.1；98.3），AUC（曲线下面积）为 0.963（0.947；0.974）。儿科患者的敏感性（87.4，48.2；98.1）估计值较低，特异性（99.2，91.6；99.9）估计值较高，与所有年龄类别相比（敏感性 95.3，91.3；97.5，特异性 96.7，93.7；98.3）。在各种指标测试中，Seeplex/Allplex 和 Amplidiag/Novodiag 的敏感性（88.9，73.8；95.8）和特异性（95.2，86；98.4）估计值最低，BDMax 的（敏感性 98.1，96.1；99 和特异性 98.5，97；99.3）最高。多重核酸检测显示出优异的准确性，可在诊断感染中发挥重要作用。
