Barzelay Aya, Levy Ran, Kohn Emmanulle, Sella Meirav, Shani Nir, Meilik Benjamin, Entin-Meer Michal, Gur Eyal, Loewenstein Anat, Barak Adiel
Dr Barzelay is a resident, Drs Levy and Kohn are Assistant Researchers, Dr Loewenstein is a Professor of Ophthalmology and Chair of the Department of Ophthalmology, and Dr Barak is a Professor of Ophthalmology and Director of the Vitreoretinal Surgery Unit in the Ophthalmology Laboratory and Department of Ophthalmology at the Tel-Aviv Sourasky Medical Center in Tel-Aviv, Israel. Dr Sella is an Assistant Researcher, Dr Meilik is an Associate Professor and Assistant Researcher, and Dr Gur is a Professor and Chair of the Department of Plastic and Reconstructive Surgery at the Tel-Aviv Sourasky Medical Center. Dr Entin-Meer is an Assistant Researcher in the Department of Cardiology at the Tel-Aviv Sourasky Medical Center.
Aesthet Surg J. 2015 Sep;35(7):NP230-40. doi: 10.1093/asj/sjv055.
Adipose tissue-derived mesenchymal stem cells (ASCs) can be isolated from subcutaneous fat harvested by tissue resection or liposuction.
The authors compared ASCs isolated by tissue resection or power-assisted liposuction (PAL) to determine whether either surgical procedure yielded ASCs with improved purity and competence that was preserved for several passages.
For this experimental study, ASCs were isolated from fat harvested by tissue resection or PAL from six patients who underwent abdominoplasty. ASCs were counted to determine cell yields, and viabilities were assessed with an amine-reactive dye and by fluorescence-activated cell sorting (FACS). Cell phenotypes were determined by immunostaining and FACS, and doubling times were calculated. Senescence ratios of the cells were detected by gene profiling and by assaying β-galactosidase activity. Multipotency was evaluated by induced differentiation analyses.
No significant differences were observed in cell numbers or viabilities of ASCs isolated following either surgical method of fat harvesting. Both populations of cultured ASCs expressed markers of mesenchymal stem cells and preserved this expression pattern through the third passage. PAL and tissue resection yielded ASCs with similar division rates, similar senescence ratios into the fourth passage, and similar capacities to differentiate into osteocytes or adipocytes.
Fat harvested by PAL or tissue resection yielded uniform cultures of ASCs with high division rates, low senescence ratios, and multipotency preserved into passages 3 and 4. Because PAL is less invasive, it may be preferable for the isolation of ASCs.
脂肪组织来源的间充质干细胞（ASCs）可从通过组织切除或抽脂获取的皮下脂肪中分离得到。
作者比较了通过组织切除或动力辅助抽脂（PAL）分离得到的ASCs，以确定这两种手术方法所获得的ASCs是否具有更高的纯度和能力，并且在传代培养数代后仍能保持这些特性。
在这项实验研究中，从6例接受腹部整形手术患者通过组织切除或PAL获取的脂肪中分离ASCs。对ASCs进行计数以确定细胞产量，并用胺反应性染料和荧光激活细胞分选（FACS）评估细胞活力。通过免疫染色和FACS确定细胞表型，并计算倍增时间。通过基因谱分析和检测β-半乳糖苷酶活性来检测细胞的衰老率。通过诱导分化分析评估多能性。
两种脂肪获取手术方法分离得到的ASCs在细胞数量或活力方面均未观察到显著差异。培养的ASCs群体均表达间充质干细胞标志物，并在传至第三代时保持这种表达模式。PAL和组织切除所获得的ASCs具有相似的分裂率、传至第四代时相似的衰老率以及相似的分化为骨细胞或脂肪细胞的能力。
通过PAL或组织切除获取的脂肪产生了均匀的ASCs培养物，具有高分裂率、低衰老率，并且在传至第3代和第4代时仍保持多能性。由于PAL的侵入性较小，它可能更适合用于ASCs的分离。
