Iyyanki Tejaswi, Hubenak Justin, Liu Jun, Chang Edward I, Beahm Elisabeth K, Zhang Qixu
From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
Aesthet Surg J. 2015 May;35(4):467-76. doi: 10.1093/asj/sju055. Epub 2015 Mar 18.
The success of an autologous fat graft depends in part on its total stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs). However, variations in the yields of ASCs and SVF cells as a result of different harvesting techniques and donor sites are poorly understood.
To investigate the effects of adipose tissue harvesting technique and donor site on the yield of ASCs and SVF cells.
Subcutaneous fat tissues from the abdomen, flank, or axilla were harvested from patients of various ages by mechanical liposuction, direct surgical excision, or Coleman's technique with or without centrifugation. Cells were isolated and then analyzed with flow cytometry to determine the yields of total SVF cells and ASCs (CD11b-, CD45-, CD34+, CD90+, D7-FIB+). Differences in ASC and total SVF yields were assessed with one-way analysis of variance. Differentiation experiments were performed to confirm the multilineage potential of cultured SVF cells.
Compared with Coleman's technique without centrifugation, direct excision yielded significantly more ASCs (P < .001) and total SVF cells (P = .007); liposuction yielded significantly fewer ASCs (P < .001) and total SVF cells (P < .05); and Coleman's technique with centrifugation yielded significantly more total SVF cells (P < .005), but not ASCs. The total number of SVF cells in fat harvested from the abdomen was significantly larger than the number in fat harvested from the flank or axilla (P < .05). Cultured SVF cells differentiated to adipocytes, osteocytes, and chondrocytes.
Adipose tissue harvested from the abdomen through direct excision or Coleman's technique with centrifugation was found to yield the most SVF cells and ASCs.
自体脂肪移植的成功部分取决于其总的基质血管成分（SVF）和脂肪来源干细胞（ASC）。然而，由于不同的采集技术和供区导致的ASC和SVF细胞产量差异尚不清楚。
研究脂肪组织采集技术和供区对ASC和SVF细胞产量的影响。
通过机械吸脂、直接手术切除或科尔曼技术（有或无离心），从不同年龄患者的腹部、侧腹或腋窝采集皮下脂肪组织。分离细胞，然后用流式细胞术分析，以确定总SVF细胞和ASC（CD11b-、CD45-、CD34+、CD90+、D7-FIB+）的产量。用单因素方差分析评估ASC和总SVF产量的差异。进行分化实验以证实培养的SVF细胞的多向分化潜能。
与未离心的科尔曼技术相比，直接切除产生的ASC（P <.001）和总SVF细胞（P =.007）明显更多；吸脂产生的ASC（P <.001）和总SVF细胞（P <.05）明显更少；离心的科尔曼技术产生的总SVF细胞明显更多（P <.005），但ASC没有明显增多。从腹部采集的脂肪中SVF细胞的总数明显大于从侧腹或腋窝采集的脂肪中的数量（P <.05）。培养的SVF细胞分化为脂肪细胞、骨细胞和软骨细胞。
发现通过直接切除或离心的科尔曼技术从腹部采集的脂肪组织产生的SVF细胞和ASC最多。
