Ridpath J F, Bayles D O, Neill J D, Falkenberg S M, Bauermann F V, Holler L, Braun L J, Young D B, Kane S E, Chase C C L
National Animal Disease Center, ARS/USDA, Ames, IA 50010, United States.
National Animal Disease Center, ARS/USDA, Ames, IA 50010, United States.
Virology. 2015 Nov;485:297-304. doi: 10.1016/j.virol.2015.07.022. Epub 2015 Aug 28.
Exposure to bovine viral diarrhea viruses (BVDV) results in acute and persistent infections. Persistent infections result from in utero exposure during the first trimester of gestation. Clinical presentation, in persistently infected cattle (PI), is highly variable. The reasons for this variation is largely unknown. The BVDV circulating in PI exist as quasispecies (swarms of individual viruses). An outbreak resulting in 34 PI cattle presented an opportunity to compare a large number of PI׳s. Methods were developed to compare the circulating viral populations within PI animals. It was found that PI animals generated in the same outbreak carry circulating viral populations that differ widely in size and diversity. Further, it was demonstrated that variation in PI viral populations could be used as a quantifiable phenotype. This observation makes it possible to test the correlation of this phenotype to other phenotypes such as growth rate, congenital defects, viral shed and cytokine expression.
接触牛病毒性腹泻病毒（BVDV）会导致急性和持续性感染。持续性感染是由妊娠头三个月子宫内接触病毒引起的。持续感染的牛（PI）的临床表现差异很大。这种差异的原因很大程度上尚不清楚。在PI中循环的BVDV以准种（单个病毒群）的形式存在。一次导致34头PI牛的疫情提供了一个比较大量PI牛的机会。已开发出方法来比较PI动物体内循环的病毒群体。研究发现，在同一疫情中产生的PI动物携带的循环病毒群体在大小和多样性上有很大差异。此外，还证明了PI病毒群体的变异可以用作可量化的表型。这一观察结果使得测试这种表型与其他表型（如生长速度、先天性缺陷、病毒脱落和细胞因子表达）之间的相关性成为可能。
