Kumar Rajesh, Grønhaug Kirsten M, Afseth Nils K, Isaksen Vidar, de Lange Davies Catharina, Drogset Jon O, Lilledahl Magnus B
Department of Physics, Norwegian University of Science and Technology (NTNU), 7491, Trondheim, Norway.
Orthopaedic Department, Levanger Hospital, Kirkegata 2, 7600, Levanger, Norway.
Anal Bioanal Chem. 2015 Oct;407(26):8067-77. doi: 10.1007/s00216-015-8979-5. Epub 2015 Aug 29.
Biomolecular changes in the cartilage matrix during the early stage of osteoarthritis may be detected by Raman spectroscopy. The objective of this investigation was to determine vibrational spectral differences among different grades (grades I, II, and III) of osteoarthritis in human osteoarthritic cartilage, which was classified according to the International Cartilage Repair Society (ICRS) grading system. Degenerative articular cartilage samples were collected during total joint replacement surgery and were classified according to the ICRS grading system for osteoarthritis. Twelve cartilage sections (4 sections of each ICRS grades I, II, and III) were selected for Raman spectroscopic analysis. Safranin-O/Fast green was used for histological staining and assignment of the Osteoarthritis Research Society International (OARSI) grade. Multivariate principal component analysis (PCA) was used for data analysis. Spectral analysis indicates that the content of disordered coil collagen increases significantly during the early progression of osteoarthritis. However, the increase was not statistically significant during later stages of the disease. A decrease in the content of proteoglycan was observed only during advanced stages of osteoarthritis. Our investigation shows that Raman spectroscopy can classify the different stage of osteoarthritic cartilage and can provide details on biochemical changes. This proof-of-concept study encourages further investigation of fresh cartilage on a larger population using fiber-based miniaturized Raman probe for the development of in vivo Raman arthroscopy as a potential diagnostic tool for osteoarthritis.
骨关节炎早期软骨基质中的生物分子变化可通过拉曼光谱检测到。本研究的目的是确定根据国际软骨修复协会（ICRS）分级系统分类的人类骨关节炎软骨不同等级（I级、II级和III级）之间的振动光谱差异。在全关节置换手术期间收集退行性关节软骨样本，并根据ICRS骨关节炎分级系统进行分类。选择十二个软骨切片（ICRS I级、II级和III级各4个切片）进行拉曼光谱分析。番红O/固绿用于组织学染色和国际骨关节炎研究协会（OARSI）分级的评定。采用多元主成分分析（PCA）进行数据分析。光谱分析表明，在骨关节炎早期进展过程中，无序卷曲胶原蛋白的含量显著增加。然而，在疾病后期，这种增加没有统计学意义。仅在骨关节炎晚期观察到蛋白聚糖含量降低。我们的研究表明，拉曼光谱可以对骨关节炎软骨的不同阶段进行分类，并能提供生化变化的详细信息。这项概念验证研究鼓励使用基于光纤的微型拉曼探针，在更大规模人群中对新鲜软骨进行进一步研究，以开发体内拉曼关节镜作为骨关节炎的潜在诊断工具。
