Padakandla Shalem Raj, Lee Gun-Woong, Chae Jong-Chan
Division of Biotechnology, Chonbuk National University, Iksan, 570-752, Republic of Korea.
Antonie Van Leeuwenhoek. 2015 Nov;108(5):1197-203. doi: 10.1007/s10482-015-0574-4. Epub 2015 Aug 29.
A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1(T), was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1(T) showed highest 16S rRNA gene sequence similarity (97.4 and 96.5%, respectively) to Paenibacillus shenyangensis A9(T) and Paenibacillus hunanensis FeL05(T), and clustered closely with the members of the family Paenibacillaceae. DNA-DNA hybridization studies revealed a genomic relatedness of 47 ± 9% with P. shenyangensis A9(T). The predominant fatty acids of strain PG1(T) were identified to be anteiso-C(15:0) (46.7%) and C(16:0) (22.7%). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol%. This polyphasic characterisation of the newly isolated strain PG1(T) justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1(T) = KCTC 33642(T) = JCM 30624(T)) is proposed.
从韩国江华岛的开垦地土壤中分离出一株杆状、革兰氏染色阳性、不运动、兼性厌氧且能水解明胶的细菌，PG1(T)菌株。PG1(T)菌株与沈阳芽孢杆菌A9(T)和湖南芽孢杆菌FeL05(T)的16S rRNA基因序列相似性最高（分别为97.4%和96.5%），并与芽孢杆菌科成员紧密聚类。DNA-DNA杂交研究表明，它与沈阳芽孢杆菌A9(T)的基因组相关性为47±9%。PG1(T)菌株的主要脂肪酸被鉴定为anteiso-C(15:0)（46.7%）和C(16:0)（22.7%）。发现双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂是主要极性脂质。基因组DNA G+C含量为47.7 mol%。新分离的PG1(T)菌株的多相特征证明将其描述为芽孢杆菌属一个新物种的代表是合理的，为此提出新名称解明芽孢杆菌（Paenibacillus gelatinilyticus sp. nov.），（模式菌株=PG1(T)=KCTC 33642(T)=JCM 30624(T)）。
