College of Resource & Civil Engineering, Northeastern University, Shenyang 110004, PR China.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):220-224. doi: 10.1099/ijs.0.060483-0. Epub 2014 Oct 16.
A Gram-stain-positive, aerobic or facultatively anaerobic, rod-shaped, non-motile, endospore-forming bacterium, strain A9(T), was isolated in 1996 from a soil sample collected under a peach tree in Qingnian Park in Shenyang, PR China, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus, and was most closely related to the type strain of Paenibacillus hunanensis with a 16S rRNA gene sequence similarity of 96.7 % and a DNA-DNA relatedness value of 51.6 %. The major polar lipids of strain A9(T) were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was MK-7 and the major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C15 : 0. The DNA G+C content was 51.9 mol%. Based on these results, it is concluded that strain A9(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus shenyangensis sp. nov. is proposed, with A9(T) ( = JCM 19307(T) = CGMCC 2040(T)) as the type strain.
一株革兰氏阳性、需氧或兼性厌氧、杆状、非运动、产芽孢的细菌，菌株 A9(T)，于 1996 年从中国辽宁省沈阳市青年公园桃树下的土壤样本中分离得到，采用多相分类法研究了其分类地位。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于类芽孢杆菌属，与类芽孢杆菌湖南亚种的 16S rRNA 基因序列相似度为 96.7%，DNA-DNA 同源性值为 51.6%，最为接近。菌株 A9(T)的主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。主要的menaquinone 为 MK-7，主要的细胞脂肪酸为anteiso-C15:0、C16:0 和 iso-C15:0。DNA G+C 含量为 51.9 mol%。基于这些结果，推断菌株 A9(T)代表了类芽孢杆菌属的一个新种，命名为沈阳类芽孢杆菌新种，其模式菌株为 A9(T)(=JCM 19307(T)=CGMCC 2040(T))。
