Liu Jie, Song Keqing, Teng Huajing, Zhang Bin, Li Wenzhu, Xue Huaijun, Yang Xingke
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China.
Acta Biochim Biophys Sin (Shanghai). 2015 Sep;47(9):741-8. doi: 10.1093/abbs/gmv070.
The Cerambycidae (longhorn beetle) is a large family of Coleoptera with xylophagous feeding habits. Cellulose digestion plays an important role in these wood-feeding insects. In this study, transcriptomic technology was used to obtain one glycoside hydrolase family 45 (GH45) cellulase and seven GH5 cellulases from Mesosa myops, a typical longhorn beetle. Analyses of expression dynamics and evolutionary relationships provided a complete description of the cellulolytic system. The expression dynamics related to individual development indicated that endogenous GH45 and GH5 cellulases dominate cellulose digestion in M. myops. Evolutionary analyses suggested that GH45 cellulase gene is a general gene in the Coleoptera Suborder Polyphaga. Evolutionary analyses also indicated that the GH5 cellulase group in Lamiinae longhorn beetles is closely associated with wood feeding. This study demonstrated that there is a complex endogenous cellulolytic system in M. myops that is dominated by cellulases belonging to two glycoside hydrolase families.
天牛科（长角甲虫）是鞘翅目中一个具有蛀木取食习性的大家族。纤维素消化在这些以木材为食的昆虫中起着重要作用。在本研究中，利用转录组技术从典型的长角甲虫云斑白条天牛中获得了一种糖苷水解酶家族45（GH45）纤维素酶和七种GH5纤维素酶。对表达动态和进化关系的分析提供了纤维素分解系统的完整描述。与个体发育相关的表达动态表明，内源性GH45和GH5纤维素酶在云斑白条天牛的纤维素消化中起主导作用。进化分析表明，GH45纤维素酶基因是多食亚目鞘翅目中的一个常见基因。进化分析还表明，天牛亚科长角甲虫中的GH5纤维素酶组与木材取食密切相关。本研究表明，云斑白条天牛中存在一个复杂的内源性纤维素分解系统，该系统由属于两个糖苷水解酶家族的纤维素酶主导。
