Fujii Yuta, Tochitani Tomoaki, Kouchi Mami, Matsumoto Izumi, Yamada Toru, Funabashi Hitoshi
Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan
Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan.
J Vet Diagn Invest. 2015 Sep;27(5):637-40. doi: 10.1177/1040638715599570. Epub 2015 Aug 28.
A male domestic ferret (Mustela putorius furo), which was purchased from outside of Japan at 13 weeks of age, was euthanized at 18 months of age because of poor health. At autopsy, the liver, spleen, and mesenteric lymph node were enlarged, and white foci were observed on the outer surface of the liver. The outer surface of the mesenteric lymph node was dark red. Histologically, granulomas were observed in the liver, spleen, bone marrow, and lymph nodes, composed mainly of aggregated epithelioid macrophages, some of which were positive to an anti-feline coronavirus (FCoV; Alphacoronavirus 1) antibody in immunohistochemistry. Mesangioproliferative glomerulonephritis was observed, and periodic acid-Schiff-positive deposits were observed along glomerular capillary walls. These deposits stained pale red with periodic acid-methenamine silver stain and red with Masson trichrome stain, and were also observed in the mesangial matrix. In affected glomeruli, glomerular capillary walls and mesangial areas were positive for anti-ferret immunoglobulin G. By electron microscopy, subepithelial and mesangial electron-dense deposits were observed consistent with immune complex deposition. The deposition of immune complexes may have been associated with FCoV infection.
一只13周龄时从日本境外购入的雄性家养雪貂（Mustela putorius furo），因健康状况不佳于18月龄时实施安乐死。尸检时，肝脏、脾脏和肠系膜淋巴结肿大，肝脏外表面可见白色病灶。肠系膜淋巴结外表面呈暗红色。组织学检查发现，肝脏、脾脏、骨髓和淋巴结中有肉芽肿，主要由聚集的上皮样巨噬细胞组成，其中一些在免疫组织化学中对抗猫冠状病毒（FCoV；甲型冠状病毒1）抗体呈阳性。观察到系膜增生性肾小球肾炎，沿肾小球毛细血管壁可见过碘酸希夫阳性沉积物。这些沉积物在用过碘酸-亚甲胺银染色时呈淡红色，用Masson三色染色时呈红色，在系膜基质中也有观察到。在受影响的肾小球中，肾小球毛细血管壁和系膜区对抗雪貂免疫球蛋白G呈阳性。通过电子显微镜观察，可见上皮下和系膜电子致密沉积物，符合免疫复合物沉积。免疫复合物的沉积可能与FCoV感染有关。
