Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark.
Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark; Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Republic of Singapore.
J Mol Biol. 2015 Nov 20;427(23):3628-45. doi: 10.1016/j.jmb.2015.08.016. Epub 2015 Aug 28.
Studies of biopsies from infectious sites, explanted tissue and medical devises have provided evidence that biofilms are the underlying cause of a variety of tissue-associated and implant-associated recalcitrant human infections. With a need for novel anti-biofilm treatment strategies, research in biofilm infection microbiology, biofilm formation mechanisms and biofilm-associated antimicrobial tolerance has become an important area in microbiology. Substantial knowledge about biofilm formation mechanisms, biofilm-associated antimicrobial tolerance and immune evasion mechanisms has been obtained through work with biofilms grown in in vitro experimental setups, and the relevance of this information in the context of chronic infections is being investigated by the use of animal models of infection. Because our current in vitro experimental setups and animal models have limitations, new advanced in vitro models developed with knowledge about the chemical landscape at infectious sites are needed.
对感染部位活检样本、移植组织和医疗设备的研究提供了证据，表明生物膜是多种与组织相关和与植入物相关的难治性人类感染的根本原因。由于需要新的抗生物膜治疗策略，生物膜感染微生物学、生物膜形成机制和生物膜相关抗菌药物耐受性的研究已成为微生物学的一个重要领域。通过在体外实验设置中培养生物膜进行的工作，已经获得了大量关于生物膜形成机制、生物膜相关抗菌药物耐受性和免疫逃避机制的知识，并且正在通过使用感染动物模型来研究这些信息在慢性感染背景下的相关性。由于我们当前的体外实验设置和动物模型存在局限性，因此需要使用有关感染部位化学环境的知识开发新的先进体外模型。
