Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA.
Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA.
Curr Opin Biotechnol. 2015 Dec;36:32-9. doi: 10.1016/j.copbio.2015.08.001. Epub 2015 Aug 28.
Phototrophs are attractive candidates for commercial lipid production. Lipid biosynthetic pathways in these organisms have been largely characterized but the mechanisms partitioning resources toward storage lipids are poorly understood. One promising strategy to study and enhance biomass lipid bioproduction in oleaginous microorganisms is to combine genome-scale metabolic modeling and genetic and metabolic engineering. Here we describe recent advances in in vitro, in vivo, and in silico manipulations of phototrophic metabolism that increase total lipid content or redirect lipid production toward more favorable products such as polyunsaturated fatty acids used as nutritional supplements or in biofuel production.
自养生物是商业脂质生产的有吸引力的候选者。这些生物体中的脂质生物合成途径已在很大程度上得到了描述，但将资源分配到储存脂质的机制还知之甚少。在产油微生物中研究和增强生物量脂质生物生产的一种很有前途的策略是结合基因组规模的代谢建模以及遗传和代谢工程。在这里，我们描述了最近在体外、体内和计算机模拟中对光合代谢的操纵的进展，这些操纵可以增加总脂质含量或使脂质生产转向更有利的产品，例如用作营养补充剂或生物燃料生产的多不饱和脂肪酸。
