Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, Baltimore, MD, 21250, USA.
Department of Agronomy and Plant Breeding, University of Guilan, Rasht, Islamic Republic of Iran.
J Ind Microbiol Biotechnol. 2020 Oct;47(9-10):845-862. doi: 10.1007/s10295-020-02290-8. Epub 2020 Jul 4.
Yarrowia lipolytica is an oleaginous yeast that has been substantially engineered for production of oleochemicals and drop-in transportation fuels. The unique acetyl-CoA/malonyl-CoA supply mode along with the versatile carbon-utilization pathways makes this yeast a superior host to upgrade low-value carbons into high-value secondary metabolites and fatty acid-based chemicals. The expanded synthetic biology toolkits enabled us to explore a large portfolio of specialized metabolism beyond fatty acids and lipid-based chemicals. In this review, we will summarize the recent advances in genetic, omics, and computational tool development that enables us to streamline the genetic or genomic modification for Y. lipolytica. We will also summarize various metabolic engineering strategies to harness the endogenous acetyl-CoA/malonyl-CoA/HMG-CoA pathway for production of complex oleochemicals, polyols, terpenes, polyketides, and commodity chemicals. We envision that Y. lipolytica will be an excellent microbial chassis to expand nature's biosynthetic capacity to produce plant secondary metabolites, industrially relevant oleochemicals, agrochemicals, commodity, and specialty chemicals and empower us to build a sustainable biorefinery platform that contributes to the prosperity of a bio-based economy in the future.
解脂耶氏酵母是一种产油脂酵母，其已被大规模工程化用于生产油脂化学品和可替代运输燃料。该酵母独特的乙酰辅酶 A/丙二酰辅酶 A 供应模式以及多功能碳利用途径使其成为一种优良的宿主，可以将低价值碳转化为高价值的次生代谢物和基于脂肪酸的化学品。扩展的合成生物学工具包使我们能够探索大量专门的代谢途径，超出了脂肪酸和基于脂质的化学品。在这篇综述中，我们将总结遗传、组学和计算工具开发的最新进展，这些进展使我们能够简化遗传或基因组修饰，用于解脂耶氏酵母。我们还将总结各种代谢工程策略，利用内源性乙酰辅酶 A/丙二酰辅酶 A/HMG-CoA 途径生产复杂的油脂化学品、多元醇、萜类化合物、聚酮化合物和大宗商品化学品。我们设想，解脂耶氏酵母将成为一个极好的微生物底盘，以扩大自然界的生物合成能力，生产植物次生代谢物、工业相关的油脂化学品、农用化学品、大宗商品和特种化学品，并使我们能够建立一个可持续的生物炼制平台，为未来基于生物的经济的繁荣做出贡献。
