Department of Microbiology Viborg-Herning, Viborg, Denmark.
Department of Microbiology Viborg-Herning, Viborg, Denmark.
Clin Microbiol Infect. 2015 Dec;21(12):1097.e1-4. doi: 10.1016/j.cmi.2015.08.006. Epub 2015 Aug 28.
Actinotignum schaalii (former named Actinobaculum schaalii) can cause urinary tract infections (UTIs) and bacteraemia, mainly in the elderly. A. schaalii is difficult to identify with conventional biochemical tests, and it is often overlooked if the urine is only cultured in ambient air. The aim of this study was to validate data from the nationwide Danish microbiology database (MiBa) with data from the laboratory information system (LIS) at the local department of microbiology in Viborg-Herning, and to evaluate the incidence rate of bacteraemia caused by A. schaalii in Denmark by using data from the MiBa. All departments of microbiology in Denmark report data to the MiBa. All microbiological samples with A. schaalii in Denmark were extracted for a period of 5 years from the MiBa and from the local LISs. All data obtained from our local LIS were also found in the MiBa, except for data on real-time PCR, which were not registered, owing to missing ID codes in the MiBa. From 2010 to 2014, there was a significant increase in the incidence rate of blood cultures with A. schaalii, from 1.8 to 6.8 cases per million, which was probably due to coincident implementation of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in routine diagnostics. We found that A. schaalii caused bacteraemia and UTIs mainly in the elderly. In conclusion, the MiBa can be a useful source of nationwide microbiological data in Denmark. Our results suggest that the incidence rate of A. schaalii as a cause of bacteraemia has been underestimated, and that culture of urine in CO2 can improve the detection of A. schaalii.
沙雷氏 Actinotignum（前称 Actinobaculum schaalii）可引起尿路感染（UTI）和菌血症，主要发生在老年人中。常规生化试验难以鉴定 A. schaalii，如果仅在环境空气中培养尿液，很容易被忽略。本研究旨在验证丹麦全国微生物学数据库（MiBa）的数据，并与 Viborg-Herning 当地微生物学系的实验室信息系统（LIS）的数据进行比较，使用 MiBa 中的数据评估丹麦由 A. schaalii引起的菌血症的发生率。丹麦所有的微生物学部门都向 MiBa 报告数据。从 MiBa 和当地的 LIS 中提取了丹麦所有的微生物样本，这些样本中都含有 A. schaalii。从我们当地的 LIS 获得的所有数据都可以在 MiBa 中找到，除了实时 PCR 数据，由于 MiBa 中缺少 ID 代码，这些数据未被登记。2010 年至 2014 年，血培养中 A. schaalii 的发病率显著增加，从每百万 1.8 例增加到 6.8 例，这可能是由于 MALDI-TOF MS 在常规诊断中的同时应用。我们发现 A. schaalii 主要引起老年人的菌血症和 UTI。总之，MiBa 可以成为丹麦全国微生物学数据的有用来源。我们的结果表明，A. schaalii 作为菌血症病因的发病率被低估了，并且在 CO2 中培养尿液可以提高 A. schaalii 的检出率。
