Májský A
Cas Lek Cesk. 1989 Dec 8;128(50):1591-3.
The author describes a new method of isolation of monocytes from peripheral blood, involving hypertonization of the blood and isolation of monocytes by centrifuging above a gradient of hypertonic Lymphoprep. The method makes it possible to obtain a monocyte suspension with at least 85% cells; there are no B lymphocytes in the suspension and the monocytes are not damaged. The quality of the thus obtained monocytes was tested by examination with monocytic sera and with HLA-DR antibodies, whereby the test was concurrently made with monocytes obtained by adherence and with B lymphocytes.
作者描述了一种从外周血中分离单核细胞的新方法，该方法包括对血液进行高渗处理，并通过在高渗淋巴细胞分离液梯度上离心来分离单核细胞。该方法能够获得细胞含量至少为85%的单核细胞悬液；悬液中不存在B淋巴细胞，且单核细胞未受损伤。通过用单核细胞血清和HLA - DR抗体进行检测，对由此获得的单核细胞质量进行了测试，同时用通过贴壁法获得的单核细胞和B淋巴细胞进行了测试。
