Modaeinama Sina, Abasi Mozhgan, Abbasi Mehran Mesgari, Jahanban-Esfahlan Rana
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(14):5697-701. doi: 10.7314/apjcp.2015.16.14.5697.
Medicinal plants, especially examples rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Punica granatum (PG) (pomegranate) is a well known fruit in this context, but its cytotoxicity in cancer cells has not been extensively studied. Here, we investigated the antiproliferative properties of a peel extract of PG from Iran in different human cancer cells.
A methanolic extract of pomegranate peel (PPE) was prepared. Total phenolic content(TPC) and total flavonoid conetnt (TFC) were determined by colorimetric assays. Antioxidant activity was determined by DPPH radical scavenging activity. The cytotoxicity of different doses of PPE (0, 5, 20, 100, 250, 500, 1000 μg/ml) was evaluated by MTT assays with A549 (lung non small cell cancer), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer), and PC-3 (prostate adenocarcinoma) cells.
Significant (P<0.01) or very significant (P<0.0001) differences were observed in comparison with negative controls at all tested doses (5-1000 μg/ml). In all studied cancer cells, PPE reduced the cell viability to values below 40%, even at the lowest doses. In all cases, IC50 was determined at doses below 5μg/ml. In this regard, MCF-7 breast adenocarcinoma cells were the most responsive cells to antiprolifreative effects of PPE with a maximum mean growth inhibition of 81.0% vs. 69.4%, 79.3% and 77.5% in SKOV3, PC-3 and A549 cells, respectively.
Low doses of PPE exert potent anti-proliferative effects in different human cancer cells and it seems that MCF-7 breast adenocarcinoma cells are the most cells and SKOV3 ovarian cancer cells the least responsive in this regard. However, the mechanisms of action need to be addressed.
药用植物，尤其是富含多酚类化合物的植物，因其抗氧化特性而被认为具有化学预防作用。在这方面，石榴（PG）是一种广为人知的水果，但其对癌细胞的细胞毒性尚未得到广泛研究。在此，我们研究了来自伊朗的石榴果皮提取物在不同人类癌细胞中的抗增殖特性。
制备石榴皮甲醇提取物（PPE）。通过比色法测定总酚含量（TPC）和总黄酮含量（TFC）。通过DPPH自由基清除活性测定抗氧化活性。采用MTT法评估不同剂量（0、5、20、100、250、500、1000μg/ml）的PPE对A549（肺非小细胞癌）、MCF-7（乳腺腺癌）、SKOV3（卵巢癌）和PC-3（前列腺腺癌）细胞的细胞毒性。
在所有测试剂量（5 - 1000μg/ml）下，与阴性对照相比，观察到显著（P<0.01）或极显著（P<0.0001）差异。在所有研究的癌细胞中，即使在最低剂量下，PPE也能将细胞活力降低至40%以下。在所有情况下，IC50均在5μg/ml以下的剂量下测定。在这方面，MCF-7乳腺腺癌细胞对PPE的抗增殖作用最敏感，最大平均生长抑制率为81.0%，而SKOV3、PC-3和A549细胞分别为69.4%、79.3%和77.5%。
低剂量的PPE在不同人类癌细胞中发挥强大的抗增殖作用，在这方面，MCF-7乳腺腺癌细胞似乎最敏感，而SKOV3卵巢癌细胞最不敏感。然而，其作用机制仍需进一步探讨。
