Jahanban-Esfahlan Akram, Modaeinama Sina, Abasi Mozhgan, Abbasi Mehran Mesgari, Jahanban-Esfahlan Rana
Department of Nursing, Faculty of Maragheh, Urmia University, Urmia, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(14):5703-7. doi: 10.7314/apjcp.2015.16.14.5703.
Medicinal plants, especially examples rich in polyphenolic compounds, have been suggested to be chemopreventive on account of their antioxidative properties. Melissa officinalis L. (MO), an aromatic and medicinal plant, is well known in thios context. However, toxicity against cancer cells has not been fully studied. Here, we investigated the selective anticancer effects of an MO extract (MOE) in different human cancer cells.
a hydro-alcoholic extract of MO was prepared and total phenolic content (TPC) and total flavonoid content (TFC) were determined by colorimetric assays. Antioxidant activity was determined by DPPH radical scavenging activity. MTT assays were used to evaluate cytotoxicity of different doses of MOE (0, 5, 20, 100, 250, 500, 1000 μg/ml) towards A549 (lung non small cell cancer cells), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer cells), and PC-3 (prostate adenocarcinoma) cells.
Significant (P<0.01) or very significant (P<0.0001) differences were observed in comparison to negative controls at all tested doses (5-1000 μg/ml). In all cancer cells, MOE reduced the cell viability to values below 33%, even at the lowest doses. In all cases, IC50 values were below 5μg/ml. The mean growth inhibition was 73.1%, 86.7%, 79.9% and 77.8% in SKOV3, MCF-7 and PC-3 and A549 cells, respectively.
Our results indicate that a hydro-alcoholic extract of MO possess a high potency to inhibit proliferation of different tumor cells in a dose independent manner, suggesting that an optimal biological dose is more important than a maximally tolerated one. Moreover, the antiprolifreative effect of MO seems to be tumor type specific, as hormone dependant cancers were more sensitive to antitumoral effects of MOE.
药用植物，尤其是富含多酚类化合物的植物，因其抗氧化特性而被认为具有化学预防作用。香蜂草（Melissa officinalis L.，简称MO）是一种芳香药用植物，在这方面广为人知。然而，其对癌细胞的毒性尚未得到充分研究。在此，我们研究了MO提取物（MOE）对不同人类癌细胞的选择性抗癌作用。
制备了MO的水醇提取物，通过比色法测定总酚含量（TPC）和总黄酮含量（TFC）。通过DPPH自由基清除活性测定抗氧化活性。采用MTT法评估不同剂量的MOE（0、5、20、100、250、500、1000μg/ml）对A549（肺非小细胞癌细胞）、MCF-7（乳腺腺癌）、SKOV3（卵巢癌细胞）和PC-3（前列腺腺癌细胞）的细胞毒性。
在所有测试剂量（5 - 1000μg/ml）下，与阴性对照相比，观察到显著（P<0.01）或非常显著（P<0.0001）的差异。在所有癌细胞中，即使在最低剂量下，MOE也将细胞活力降低至33%以下。在所有情况下，IC50值均低于5μg/ml。在SKOV3、MCF-7、PC-3和A549细胞中，平均生长抑制率分别为73.1%、86.7%、79.9%和77.8%。
我们的结果表明，MO的水醇提取物具有高效抑制不同肿瘤细胞增殖的能力，且呈剂量非依赖性，这表明最佳生物学剂量比最大耐受剂量更重要。此外，MO的抗增殖作用似乎具有肿瘤类型特异性，因为激素依赖性癌症对MOE的抗肿瘤作用更敏感。
