Park Jung Woo, Park Yoojin, Kim Byeang Hyean
Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea.
Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea.
Bioorg Med Chem Lett. 2015 Oct 15;25(20):4597-600. doi: 10.1016/j.bmcl.2015.08.052. Epub 2015 Aug 20.
We have constructed a simple and efficient system-based on quencher-free molecular aptamer beacons (QF-MABs)-for probing ATP. In the absence of ATP, the fluorescence of a pyrene fluorophore on the loop position (15 nucleotides from the 5' end) of the optimal QF-MAB was quenched by the neighboring nucleobases; in its presence, fluorescence was recovered, due to a conformational change in the secondary structure of the QF-MAB.
我们构建了一个基于无淬灭剂分子适配体信标（QF-MABs）的简单高效系统，用于探测ATP。在没有ATP的情况下，最佳QF-MAB环位置（从5'端起15个核苷酸）上芘荧光团的荧光被相邻的核碱基淬灭；在有ATP的情况下，由于QF-MAB二级结构的构象变化，荧光得以恢复。
