Torres-Cuevas Isabel, Millán Iván, Asensi Miguel, Vento Máximo, Oger Camille, Galano Jean-Marie, Durand Thierry, Ortega Ángel L
Neonatal Research Group, Health Research Institute La Fe, Av. Fernando Abril Martorell 106, 46026 Valencia, Spain.
Department of Physiology, Faculty of Pharmacy, University of Valencia, Vicente Andrés Estellés Av. s/n, 46100 Burjassot, Spain.
Antioxidants (Basel). 2021 Jan 23;10(2):168. doi: 10.3390/antiox10020168.
The loss of redox homeostasis induced by hyperglycemia is an early sign and key factor in the development of diabetic retinopathy. Due to the high level of long-chain polyunsaturated fatty acids, diabetic retina is highly susceptible to lipid peroxidation, source of pathophysiological alterations in diabetic retinopathy. Previous studies have shown that pterostilbene, a natural antioxidant polyphenol, is an effective therapy against diabetic retinopathy development, although its protective effects on lipid peroxidation are not well known. Plasma, urine and retinas from diabetic rabbits, control and diabetic rabbits treated daily with pterostilbene were analyzed. Lipid peroxidation was evaluated through the determination of derivatives from arachidonic, adrenic and docosahexaenoic acids by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Diabetes increased lipid peroxidation in retina, plasma and urine samples and pterostilbene treatment restored control values, showing its ability to prevent early and main alterations in the development of diabetic retinopathy. Through our study, we are able to propose the use of a derivative of adrenic acid, 17()-10---Δ-11-dihomo-IsoF, for the first time, as a suitable biomarker of diabetic retinopathy in plasmas or urine.
高血糖诱导的氧化还原稳态失衡是糖尿病视网膜病变发生发展的早期迹象和关键因素。由于长链多不饱和脂肪酸水平较高，糖尿病视网膜极易发生脂质过氧化，这是糖尿病视网膜病变病理生理改变的根源。先前的研究表明，紫檀芪作为一种天然抗氧化多酚，是对抗糖尿病视网膜病变发展的有效疗法，尽管其对脂质过氧化的保护作用尚不明确。对糖尿病兔、正常对照兔以及每日用紫檀芪治疗的糖尿病兔的血浆、尿液和视网膜进行了分析。通过超高效液相色谱-串联质谱法测定花生四烯酸、肾上腺酸和二十二碳六烯酸的衍生物来评估脂质过氧化。糖尿病会增加视网膜、血浆和尿液样本中的脂质过氧化，而紫檀芪治疗可使其恢复至对照值，表明其能够预防糖尿病视网膜病变发展过程中的早期及主要改变。通过我们的研究，我们首次能够提出将肾上腺酸衍生物17()-10---Δ-11-二高异前列腺素F用作血浆或尿液中糖尿病视网膜病变的合适生物标志物。
