内镜超声引导下细针穿刺胃肠道实性肿瘤病变的细胞块制作方法
Cell-block procedure in endoscopic ultrasound-guided-fine-needle-aspiration of gastrointestinal solid neoplastic lesions.
作者信息
Ieni Antonio, Barresi Valeria, Todaro Paolo, Caruso Rosario Alberto, Tuccari Giovanni
机构信息
Antonio Ieni, Valeria Barresi, Rosario Alberto Caruso, Giovanni Tuccari, Department of Human Pathology "Gaetano Barresi", Section of Anatomic Pathology, University of Messina, Azienda Ospedaliera Universitaria "Gaetano Martino", 98125 Messina, Italy.
出版信息
World J Gastrointest Endosc. 2015 Aug 25;7(11):1014-22. doi: 10.4253/wjge.v7.i11.1014.
Abstract
In the present review we have analyzed the clinical applications of endoscopic ultrasound-guided-fine-needle-aspiration (EUS-FNA) and the methodological aspects obtained by cell-block procedure (CBP) in the diagnostic approach to the gastrointestinal neoplastic pathology. CBP showed numerous advantages in comparison to the cytologic routine smears; in particular, better preservation of cell architecture, achievement of routine haematoxylin-eosin staining equivalent to histological slides and possibility to perform immunohistochemistry or molecular analyses represented the most evident reasons to choose this method. Moreover, by this approach, the differential diagnosis of solid gastrointestinal neoplasias may be more easily achieved and the background of contaminant non-neoplastic gastrointestinal avoided. Finally, biological samples collected by EUS-FNA CBP-assisted should be investigated in order to identify and quantify further potential molecular markers.
摘要
在本综述中，我们分析了内镜超声引导下细针穿刺活检（EUS-FNA）的临床应用以及通过细胞块制作程序（CBP）在胃肠道肿瘤病理学诊断方法中获得的方法学方面的内容。与常规细胞学涂片相比，CBP显示出许多优势；特别是，细胞结构保存更好、能实现与组织学切片相当的常规苏木精-伊红染色，以及能够进行免疫组织化学或分子分析，这些都是选择该方法的最明显原因。此外，通过这种方法，可以更轻松地实现胃肠道实体瘤的鉴别诊断，并避免非肿瘤性胃肠道污染物的干扰。最后，应对通过EUS-FNA CBP辅助收集的生物样本进行研究，以识别和量化更多潜在的分子标志物。
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