Medeiros Raphael Corrêa, Daniel Luiz Antonio
Department of Agronomic and Environmental Sciences, Federal University of Santa Maria, Linha 7 de Setembro, BR 386, Km 40, 98400-000, Frederico Westphalen - Rio Grande do Sul, Brazil E-mail:
Hydraulics and Sanitation Department, São Carlos School of Engineering, University of São Paulo, Av. Trabalhador São-Carlense, 400, 13566-590, São Carlos - São Paulo, Brazil.
J Water Health. 2015 Sep;13(3):811-8. doi: 10.2166/wh.2015.228.
More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wastewater samples using ColorSeed®. Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries.
为了推进与贾第虫和隐孢子虫（卵）囊的灭活、去除、定量及物种区分相关的研究，需要更精确的方法从废水中回收这些（卵）囊。本研究应用不同方法，使用ColorSeed®从废水样本中回收最大数量的（卵）囊。免疫磁分离主要有助于在中低浊度样本中捕获卵囊。三重离心法在原废水中对贾第虫囊和隐孢子虫卵囊的回收率分别达到85%和20%，在二级处理出水中分别为62.5%和17.5%。对于低浊度处理出水，膜过滤对贾第虫囊的回收率达到67.5%，对隐孢子虫卵囊为22.5%。简单、快速且低成本的方法对样本的处理不多，可能会很有用，尤其是在发展中国家。
