阿根廷布宜诺斯艾利斯一家中心研究：儿童和青少年的咽峡部脑膜炎奈瑟菌携带情况。
Oropharyngeal meningococcal carriage in children and adolescents, a single center study in Buenos Aires, Argentina.
机构信息
Epidemiology Division, Dr Ricardo Gutiérrez Children's Hospital, Buenos Aires, Argentina.
Clinical Microbiology Service, INEI-ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina.
出版信息
PLoS One. 2021 Mar 29;16(3):e0247991. doi: 10.1371/journal.pone.0247991. eCollection 2021.
BACKGROUND
Neisseria meningitidis (Nm) pharyngeal carriage is a necessary condition for invasive disease. We present the first carriage study in children in Buenos Aires, Argentina, considering 2017 as a transition year. Aims: to assess the rate of Nm carriage, to determine genogroup, clonal complex and outer membrane protein distribution, to determine carriage risk factors by age.
METHODS
Cross-sectional study including children 1-17 yrs, at Ricardo Gutiérrez Children's Hospital in Buenos Aires 2017. Oro-pharyngeal swabs were taken and cultured within a short time after collection. Genogroup was determined by PCR and clonal complex by MLST. Categorical variables were analyzed.
RESULTS
A total of 1,751 children were included. Group 1: 943 children 1-9 yrs, 38 Nm were isolated; overall carriage 4.0%. Genogroup distribution: B 26.3%, W 5.3%, Y 2.6%, Z 5.3%, other groups 7.9% and capsule null (cnl) 52.6%. Participating in extracurricular activities was the only independent predictor of Nm carriage. Group 2: 808 children 10-17 yrs, 76 Nm were isolated; overall carriage 9.4%. Genogroup distribution: B 19.7%, C 5.3%, W 7.9%, Y 9.2%, Z 5.3%, other groups 7.9% and cnl 44.7%. Independent predictors of carriage: attending pubs/night clubs and passive smoking (adjusted OR: 0.55, 95%CI = 0.32-0.93; p = 0.025).
CONCLUSIONS
Overall carriage was higher in 10-17 yrs. The isolates presenting the cnl locus were prevalent in both age groups and genogroup B was the second most frequent.
背景
脑膜炎奈瑟菌（Nm）咽部携带是侵袭性疾病的必要条件。我们报告了阿根廷布宜诺斯艾利斯首次对儿童进行的携带研究，将 2017 年视为过渡年。目的：评估 Nm 携带率，确定基因群、克隆复合体和外膜蛋白分布，按年龄确定携带风险因素。
方法
包括 2017 年在布宜诺斯艾利斯 Ricardo Gutiérrez 儿童医院的 1-17 岁儿童在内的横断面研究。在采集后短时间内采集口咽拭子并进行培养。通过 PCR 确定基因群，通过 MLST 确定克隆复合体。分析分类变量。
结果
共纳入 1751 名儿童。第 1 组：943 名 1-9 岁儿童，分离出 38 株 Nm，总携带率为 4.0%。基因群分布：B 26.3%、W 5.3%、Y 2.6%、Z 5.3%、其他组 7.9%和荚膜缺失（cnl）52.6%。参加课外活动是 Nm 携带的唯一独立预测因素。第 2 组：808 名 10-17 岁儿童，分离出 76 株 Nm，总携带率为 9.4%。基因群分布：B 19.7%、C 5.3%、W 7.9%、Y 9.2%、Z 5.3%、其他组 7.9%和 cnl 44.7%。携带的独立预测因素：光顾酒吧/夜总会和被动吸烟（调整后的 OR：0.55，95%CI=0.32-0.93；p=0.025）。
结论
10-17 岁儿童总体携带率较高。携带 cnl 基因座的分离株在两个年龄组中均很常见，B 基因群是第二常见的基因群。
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