Liu Chao, Hao Qing-xiu, Jin Yan, Huang Lu-qi, Kang Li-ping, Guo Lan-ping
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1710-7.
To analysis the differences between Tripterygium wilfordii and T. hypoglaucum, specimens of their leaves were collected from five production regions and analyzed by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS). The data were analyzed by multivariate statistical method, such as hierarchical cluster analysis (HCA) principal component analysis (PCA) and orthogonal signal correction partial least square discrimination (OPLS-DA). Potential markers with VIP values above 5.0 and corresponding r values above 0.85, were selected and further tested by combining mann-Whitney nonparametric. Those with P < 0.001 and AUC = 1 were confirmed as metabolite markers to discriminate them from each other. Results revealed that the two species were obviously different in their leaf metabolites. Based on their mass spectra, 23 potential metabolite markers were identified to distinguish T. wilfordii from T. hypoglaucum.
为分析雷公藤与昆明山海棠之间的差异，从五个产地采集了它们的叶片标本，并采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-Q-TOF-MS）进行分析。数据采用多元统计方法进行分析，如层次聚类分析（HCA）、主成分分析（PCA）和正交信号校正偏最小二乘判别分析（OPLS-DA）。选择VIP值大于5.0且相应r值大于0.85的潜在标志物，并结合曼-惠特尼非参数检验进行进一步测试。P<0.001且AUC = 1的标志物被确认为可区分二者的代谢物标志物。结果表明，这两个物种的叶片代谢物存在明显差异。基于它们的质谱图，鉴定出23个潜在的代谢物标志物以区分雷公藤与昆明山海棠。
