Dong Zi-bo, Li Chao, Shao Jian-guo
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1747-50.
To establish an HPLC method for the content determination of baicalin, wogonin, chlorogenic acid, caffeic acid, cichoric acid, corynoline and adenosine in Pudilan Xiaoyan oral liquid.
The analysis was performed on a Phenomenex Luna C18 column (4.6 mm x 250 mm, 5 µm) with a gradient mobile phase of methanol-0.1% trifluoroacetic acid solution system at flow rate of 1.0 mL · min(-1). The detective wavelength was at 280 nm. The column temperature was 30 °C.
The standard curves of seven studied components show good linearity in their concentration ranges with r ≥ 0.999 6. The average recovery was 98.73%-102.1% with RSD less than 2.6%.
The method is rapid, simple and accurate, and can be applied for the quality control of Pudilan Xiaoyan oral liquid.
建立一种高效液相色谱法测定蒲地蓝消炎口服液中黄芩苷、汉黄芩素、绿原酸、咖啡酸、菊苣酸、紫堇灵和腺苷的含量。
采用菲罗门Luna C18柱（4.6 mm×250 mm，5 µm），以甲醇-0.1%三氟乙酸溶液为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹，检测波长为280 nm，柱温为30℃。
7种被测成分的标准曲线在各自浓度范围内线性关系良好，r≥0.999 6，平均回收率为98.73% - 102.1%，相对标准偏差小于2.6%。
该方法快速、简便、准确，可用于蒲地蓝消炎口服液的质量控制。
