[高效液相色谱法同时测定蒲地蓝消炎口服液中7种成分]
[Simultaneous determination of seven components in Pudilan Xiaoyan oral liquid by HPLC].
作者信息
Dong Zi-bo, Li Chao, Shao Jian-guo
出版信息
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1747-50.
OBJECTIVE
To establish an HPLC method for the content determination of baicalin, wogonin, chlorogenic acid, caffeic acid, cichoric acid, corynoline and adenosine in Pudilan Xiaoyan oral liquid.
METHOD
The analysis was performed on a Phenomenex Luna C18 column (4.6 mm x 250 mm, 5 µm) with a gradient mobile phase of methanol-0.1% trifluoroacetic acid solution system at flow rate of 1.0 mL · min(-1). The detective wavelength was at 280 nm. The column temperature was 30 °C.
RESULT
The standard curves of seven studied components show good linearity in their concentration ranges with r ≥ 0.999 6. The average recovery was 98.73%-102.1% with RSD less than 2.6%.
CONCLUSION
The method is rapid, simple and accurate, and can be applied for the quality control of Pudilan Xiaoyan oral liquid.
目的
建立一种高效液相色谱法测定蒲地蓝消炎口服液中黄芩苷、汉黄芩素、绿原酸、咖啡酸、菊苣酸、紫堇灵和腺苷的含量。
方法
采用菲罗门Luna C18柱（4.6 mm×250 mm，5 µm），以甲醇-0.1%三氟乙酸溶液为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹，检测波长为280 nm，柱温为30℃。
结果
7种被测成分的标准曲线在各自浓度范围内线性关系良好，r≥0.999 6，平均回收率为98.73% - 102.1%，相对标准偏差小于2.6%。
结论
该方法快速、简便、准确，可用于蒲地蓝消炎口服液的质量控制。
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