反相高效液相色谱法测定石韦中绿原酸和圣草酚-7-O-β-D-葡萄糖醛酸苷的含量
[Determination of chlorogenic acid and eriodictyol-7-O-beta-D-glucuronide in Pyrrosia by RP-HPLC].
作者信息
Ma Chen, Zhou Yuan, Liu Ai-ru
机构信息
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
出版信息
Yao Xue Xue Bao. 2003 Apr;38(4):286-9.
AIM
To develop a method for determination of chlorogenic acid and eriodictyol-7-O-beta-D-glucuronide in Pyrrosia of different species and different places of origin by RP-HPLC.
METHODS
Chromatography was performed using a C18 column with mobile phase of methanol-water-phosphoric acid (50:200:0.2). The monitoring wavelength was 284 nm.
RESULTS
The linear ranges were 0.01-5.0 micrograms (r = 0.9997) and 0.004-5.0 micrograms (r = 0.9997), the recoveries were 97.1% (n = 8, RSD = 2.7%) and 98.8% (n = 9, RSD = 2.5%) for chlorogenic acid and eriodictyol-7-O-beta-D-glucuronide, respectively.
CONCLUSION
The method was employed to the analysis of 21 samples of Pyrrosia. The contents of compounds vary greatly depending on the species used, place of collection and time of harvesting. The HPLC method is sensitive, rapid and can be used to control the quality of Pyrrosia and to guide reasonable season of harvesting.
目的
建立用反相高效液相色谱法测定不同种源石韦中绿原酸和圣草酚-7-O-β-D-葡萄糖醛酸苷的方法。
方法
采用C18柱，以甲醇-水-磷酸（50:200:0.2）为流动相进行色谱分析。监测波长为284nm。
结果
绿原酸和圣草酚-7-O-β-D-葡萄糖醛酸苷的线性范围分别为0.01 - 5.0μg（r = 0.9997）和0.004 - 5.0μg（r = 0.9997），回收率分别为97.1%（n = 8，RSD = 2.7%）和98.8%（n = 9，RSD = 2.5%）。
结论
该方法用于分析21份石韦样品。化合物的含量因所用物种、采集地点和采收时间的不同而有很大差异。高效液相色谱法灵敏、快速，可用于控制石韦的质量并指导合理采收季节。
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