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Isolation of the Inositol Phosphoceramide Synthase Gene (AUR1) from Stress-Tolerant Yeast Pichia kudriavzevii.

作者信息

Yoo Boung-Hyuk, Kim Myoung-Dong

机构信息

Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2015 Nov;25(11):1902-7. doi: 10.4014/jmb.1508.08019.

DOI:10.4014/jmb.1508.08019
PMID:26323269
Abstract

This study is the first report of the entire nucleotide sequence of an inositol phosphoceramide synthase gene from the stress-tolerant yeast Pichia kudriavzevii (PkAUR1). Sequence analysis revealed an open reading frame that spans 1,443 bp and encodes a 480-amino-acid-residue protein with the highest sequence similarity (41.7%) to Aur1 from Spathaspora passalidarum. A phenotypic assay with transformed S. cerevisiae and P. kudriavzevii indicated that two amino acid residues, Phe166 and Gly249, play crucial roles in the resistance to aureobasidin A, which is consistent with previous reports for other fungal Aur1s. The GenBank Accession No. for PkAUR1 is KP729614.

摘要

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