Wood Paul L, Le Annie, Palazzolo Dominic L
Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, 6965 Cumberland Gap Pkwy., Harrogate, TN 37752, USA.
Clinical Training Program, DeBusk College of Osteopathic Medicine, Lincoln Memorial University, 6965 Cumberland Gap Pkwy., Harrogate, TN 37752, USA.
Metabolites. 2024 Apr 20;14(4):240. doi: 10.3390/metabo14040240.
The oral cavity contains a vast array of microbes that contribute to the balance between oral health and disease. In addition, oral bacteria can gain access to the circulation and contribute to other diseases and chronic conditions. There are a limited number of publications available regarding the comparative lipidomics of oral bacteria and fungi involved in the construction of oral biofilms, hence our decision to study the lipidomics of representative oral bacteria and a fungus. We performed high-resolution mass spectrometric analyses (<2.0 ppm mass error) of the lipidomes from five Gram-positive commensal bacteria: , , , , and ; five Gram-positive opportunistic bacteria: , , , , and ; seven Gram-negative opportunistic bacteria: , , , , , and ; and one fungus: . Our mass spectrometric analytical platform allowed for a detailed evaluation of the many structural modifications made by microbes for the three major lipid scaffolds: glycerol, sphingosine and fatty acyls of hydroxy fatty acids (FAHFAs).
口腔中存在大量微生物，它们对口腔健康与疾病之间的平衡起着重要作用。此外，口腔细菌可进入血液循环并引发其他疾病和慢性病症。目前关于参与口腔生物膜形成的口腔细菌和真菌的比较脂质组学的出版物数量有限，因此我们决定研究代表性口腔细菌和一种真菌的脂质组学。我们对五种革兰氏阳性共生菌： 、 、 、 、 ；五种革兰氏阳性机会致病菌： 、 、 、 、 ；七种革兰氏阴性机会致病菌： 、 、 、 、 、 ；以及一种真菌： 的脂质组进行了高分辨率质谱分析（质量误差<2.0 ppm）。 我们的质谱分析平台能够详细评估微生物对三种主要脂质支架：甘油、鞘氨醇和羟基脂肪酸（FAHFAs）的脂肪酰基所做的许多结构修饰。
