Gewartowska Magdalena, Olszewski Waldemar L, Buyanowskaya Olga, Frontczak-Baniewicz Malgorzata
Department of Surgical Research and Transplantology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland; Department of Human Epigenetics, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
Department of Surgical Research and Transplantology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland; Department of Human Epigenetics, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland; Department of Gastrointestinal and Transplantation Surgery and Clinical Laboratory, Central Clinical Hospital, Ministry of Internal Affairs, Warsaw, Poland.
J Surg Res. 2016 Jan;200(1):374-86. doi: 10.1016/j.jss.2015.07.046. Epub 2015 Aug 5.
Autologous venous grafts generally give best results for arterial bypass grafting in cases of arterial stenosis. When no suitable venous graft can be found, synthetic prosthetic graft may be an alternative. Prostheses are easily accessible but susceptible to infection. In these cases, the replacement of infected prosthesis by the human arterial allograft is the best treatment option. The question arises whether we could prepare a graft meeting mechanical conditions of an artery immunologically inert and resistant to bacterial infection.
LEW and BN rat aortic segments were placed in dehydrated sodium chloride and stored for 1 to 12 mo. Then, they were transplanted orthotopically as aortic grafts for 3 to 15 mo in syngenic and allogenic combination. No immunosuppression was used. Patency, pulsation, and frequency of development of aneurysms were studied. The tensile strength and maximum intraluminal pressures were measured. Morphology of grafts was evaluated on histology and electron microscopy. The endothelial and infiltrating cells were identified.
Transplanted allogeneic aortic grafts preserved in anhydrous sodium chloride up to 12 mo remained patent for 15 mo. Hypertrophy of intima with endothelial cells lining the inner surface and single muscle cells between elastic fibers were seen. Normal structure of collagen and elastic fibers was maintained. Only minor-host mononuclear infiltrates were seen around the preserved allografts.
Rat aortas preserved in anhydrous sodium chloride retain patency and function even 15 mo after transplantation. Such grafts retain their wall structure and evoke only minor recipient reaction. Our results confirm that anhydrous sodium chloride may be used for arterial grafts preservation. Low immunogenicity is additional advantage.
在动脉狭窄病例中，自体静脉移植物通常为动脉旁路移植提供最佳效果。当找不到合适的静脉移植物时，合成人工血管移植物可能是一种替代选择。人工血管移植物容易获取，但易受感染。在这些情况下，用人同种异体动脉移植物替换感染的人工血管移植物是最佳治疗选择。问题是我们是否能够制备出一种满足动脉机械条件、免疫惰性且抗细菌感染的移植物。
将LEW和BN大鼠的主动脉段置于脱水氯化钠中并保存1至12个月。然后，将它们原位移植为主动脉移植物，进行3至15个月的同基因和异基因组合移植。未使用免疫抑制。研究通畅率、搏动情况以及动脉瘤形成频率。测量拉伸强度和最大腔内压力。通过组织学和电子显微镜评估移植物的形态。鉴定内皮细胞和浸润细胞。
保存在无水氯化钠中长达12个月的移植异体主动脉移植物在15个月内保持通畅。可见内膜肥大，内表面有内皮细胞衬里，弹性纤维之间有单个肌细胞。胶原纤维和弹性纤维的正常结构得以维持。在保存的异体移植物周围仅可见少量宿主单核细胞浸润。
保存在无水氯化钠中的大鼠主动脉即使在移植后15个月仍保持通畅和功能。此类移植物保留其壁结构，仅引起轻微的受体反应。我们的结果证实无水氯化钠可用于动脉移植物保存。低免疫原性是额外的优势。
