Hachida M, Hanayama N, Bonkohara Y, Nishinaka T, Ookado A, Hoshi H, Nonoyama M, Koyanagi H
Department of Cardiovascular Surgery, Heart Institute of Japan, Tokyo Women's Medical College.
Nihon Kyobu Geka Gakkai Zasshi. 1994 Aug;42(8):1171-7.
The surgical replacement of human vessels using fresh allografts (homograft) has been reported as useful means. Nevertheless, little is known about preservation effect and immunological consequences using TC199 solution. In this study, defined inbred strains of rats were used. The aorta was extracted from Buffalo rats and preserved for 1, 2, and 3 weeks in TC199 solution at 4 degrees C. The preserved graft was then implanted into the Buffalo rats heterotopically, in the abdominal aorta, in the autotransplant group (AUTO) and into the Lewis rat in the allotransplant group (ALLO). The graft patency and histology including immunohistochemical stain (IgG, IgM, and C3 complement) were assessed at 2 weeks after transplantation. In AUTO, the patency was 100% with grafts 1 and 2 weeks' preservation, and 80% with 3 weeks' preservation. In the ALLO, patency was 100% with grafts with one week's preservation, 80% in those with 2 weeks' preservation and 0% those with in 3 weeks' preservation. Histology revealed that fibroblasts and endothelial cells were significantly deteriorated in 3 weeks' preservation in auto transplantation, however, they were well-maintained in 2 weeks' preservation. In the allo-transplantation group, histological deterioration began after 2 weeks preservation. Immunohistochemical staining showed significant deposits of IgG, IgM and C3 in ALLO. Significant infiltration of antibodies was seen in deteriorated tissue due to prolongation of the preservation periods. These results suggested that freshly preserved allograft was antigenic and 2 weeks preservation using TC199 solution was possible in the rat model.
据报道，使用新鲜同种异体移植物（同种移植）进行人体血管的外科置换是一种有效的方法。然而，关于使用TC199溶液的保存效果和免疫后果知之甚少。在本研究中，使用了特定的近交系大鼠。从布法罗大鼠中提取主动脉，并在4℃下于TC199溶液中保存1、2和3周。然后将保存的移植物异位植入布法罗大鼠的腹主动脉中，自体移植组（AUTO）以及同种异体移植组（ALLO）的刘易斯大鼠体内。在移植后2周评估移植物通畅情况和组织学，包括免疫组织化学染色（IgG、IgM和C3补体）。在自体移植组中，保存1周和2周的移植物通畅率为100%，保存3周的为80%。在同种异体移植组中，保存1周的移植物通畅率为100%，保存2周的为80%，保存3周的为0%。组织学显示，在自体移植中，保存3周时成纤维细胞和内皮细胞明显恶化，然而，在保存2周时它们保持良好。在同种异体移植组中，保存2周后开始出现组织学恶化。免疫组织化学染色显示同种异体移植组中有大量IgG、IgM和C3沉积。由于保存期延长，在恶化的组织中可见明显的抗体浸润。这些结果表明，新鲜保存的同种异体移植物具有抗原性，在大鼠模型中使用TC199溶液保存2周是可行的。
