Gewartowska M, Olszewski W L
Department of Surgical Research and Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw, Poland.
Transplant Proc. 2007 Nov;39(9):2917-9. doi: 10.1016/j.transproceed.2007.08.051.
There is a desperate need for preserved human arteries to be transplanted in infected areas of ischemic lower limbs. Cryopreserved arteries are fragile evoking strong allogeneic reactions. We have been searching for a preservation method that spares arterial wall structure and decreases its antigenicity. LEW and BN rat segments of aorta were harvested, immersed in pulverized dehydrated sodium chloride, and stored for 1 week to 3 months. Thereafter, they were desalinated and transplanted side-to-side to the abdominal aorta and side-to-side to the aorta-IVC (AV fistula). We performed staining for actin, elastic fibers, collagen (trichrome), CD31, endothelial cells (HIS52), MHC class II. Popliteal lymph node reactions were tested after subcutaneous implantation of an aortic fragment into the paw. We measured tensile strength and maximum intraluminal pressures. The preserved fragments of syngeneic aorta retained their cellular, stromal, and molecular structures. After transplantation, they did not evoke any reaction around the graft. The allogeneic fragments brought about only minor recipient responses, remaining patent and pulsatile for 3 months. The tensile strength and maximum intraluminal pressures did not significantly differ from freshly harvested, transplanted aortic segments.
迫切需要将保存的人体动脉移植到缺血性下肢的感染区域。冷冻保存的动脉很脆弱，会引发强烈的同种异体反应。我们一直在寻找一种能保留动脉壁结构并降低其抗原性的保存方法。采集LEW和BN大鼠的主动脉段，浸入粉碎的脱水氯化钠中，并保存1周至3个月。之后，将它们脱盐，并与腹主动脉进行端侧吻合移植以及与主动脉-下腔静脉进行端侧吻合（动静脉瘘）。我们对肌动蛋白、弹性纤维、胶原蛋白（三色染色）、CD31、内皮细胞（HIS52）、MHC II类进行了染色。将主动脉片段皮下植入爪部后，测试腘淋巴结反应。我们测量了拉伸强度和最大腔内压力。同基因主动脉的保存片段保留了其细胞、基质和分子结构。移植后，它们在移植物周围未引发任何反应。异基因片段仅引起受体轻微反应，在3个月内保持通畅和搏动。拉伸强度和最大腔内压力与新鲜采集并移植的主动脉段相比无显著差异。
