Ogedengbe John Onimisi, Adelaiye Alexander Babatunde, Mohammed Aliyu, Ayo Joseph Olusegun, Odili Augustine Nonso, Adeyemi Olusoji Matthew, Akeju Stella, Peter Philomina
Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Abuja, Abuja 901001, Nigeria; Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria 810000, Nigeria.
Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria 810000, Nigeria.
Pathophysiology. 2015 Dec;22(4):183-7. doi: 10.1016/j.pathophys.2015.08.001. Epub 2015 Aug 22.
This study was aimed at finding a relationship between pain modulators in the blood and physiological pain in apparently healthy Nigerians. It also aimed at establishing a pilot study for finding reference values for plasma levels of substance P, serotonin and tryptophan for the first time among Nigerians. Volunteers were made up of 110 residents of Abuja, aged between 21 and 50 years. Cold pressor test was used to induce pain assessing pain intensity, threshold and tolerance. ELISA was used to assay for plasma substance P, serotonin and tryptophan. Pain parameters from cold pressor test were correlated with plasma pain modulators measured. Results from cold pressor test revealed pain intensity to be 5.79±0.25cm, pain threshold 28.77±2.32s and pain tolerance 143.62±24.39s. Blood plasma level of substance P was 116.52±20.53pg/mL, serotonin 454.18±30.16ng/mL and tryptophan 12.77±0.67μg/mL. There was negative correlation between pain threshold and plasma substance P, pain tolerance and plasma substance P and pain threshold and plasma serotonin. There was however a positive correlation between pain intensity and plasma serotonin. In conclusion, the regression formulas may aid in using cold pressor test to predict blood substance levels of the measured pain modulators in a low resource setting like Nigeria where ELISA test is very expensive.
本研究旨在探寻健康尼日利亚人体内血液中的疼痛调节因子与生理性疼痛之间的关系。该研究还旨在开展一项初步研究，首次在尼日利亚人中确定P物质、血清素和色氨酸血浆水平的参考值。志愿者由110名年龄在21至50岁之间的阿布贾居民组成。采用冷加压试验诱发疼痛，评估疼痛强度、阈值和耐受性。采用酶联免疫吸附测定法检测血浆中的P物质、血清素和色氨酸。将冷加压试验的疼痛参数与所测血浆疼痛调节因子进行关联分析。冷加压试验结果显示，疼痛强度为5.79±0.25厘米，疼痛阈值为28.77±2.32秒，疼痛耐受性为143.62±24.39秒。血浆中P物质水平为116.52±20.53皮克/毫升，血清素为454.18±30.16纳克/毫升，色氨酸为12.77±0.67微克/毫升。疼痛阈值与血浆P物质、疼痛耐受性与血浆P物质以及疼痛阈值与血浆血清素之间呈负相关。然而，疼痛强度与血浆血清素之间呈正相关。总之，在像尼日利亚这样酶联免疫吸附测定试验非常昂贵的资源匮乏地区，回归公式可能有助于利用冷加压试验预测所测疼痛调节因子的血液物质水平。
