Akbulut Huseyin, Bozokalfa Guliz, Asker Duygu N, Demir Bilal, Guler Emine, Odaci Demirkol Dilek, Timur Suna, Yagci Yusuf
Istanbul Technical University , Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey.
Ege University Faculty of Science Biochemistry Department 35100 Bornova-Izmir, Turkey.
ACS Appl Mater Interfaces. 2015 Sep 23;7(37):20612-22. doi: 10.1021/acsami.5b04967. Epub 2015 Sep 15.
In the ever-expanding field of conducting polymer research, functionalized graft hybrid copolymers have gained considerable interest in the biomedical engineering and biosensing applications, particularly. In the present work, a new biosensor based on conducting graft copolymer for the detection of phenolic compounds was developed. Thereby, a robust and novel material, namely "polythiophene-g-poly(ethylene glycol) with lateral amino groups" (PT-NH2-g-PEG) hybrid conducting polymer was synthesized via Suzuki condensation polymerization and characterized with (1)H NMR analysis, UV-vis spectroscopy, gel permeation chromatography (GPC) and fluorescence spectroscopy. PT-NH2-g-PEG architecture was then applied as an immobilization matrix to accomplish extended biosensing function. In a typical process, Laccase was utilized as a model enzyme for the detection of phenolic compounds. Detailed surface characterization of PT-NH2-g-PEG/Lac was performed by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, fluorescence microscopy and scanning electron microscopy measurements. Optimum pH and polymer amount were found to be pH 6.5 and 0.5 mg polymer, respectively, with the linear range of 0.0025-0.05 mM and 132.45 μA/mM sensitivity. The kinetic parameters of PT-NH2-g-PEG/Lac are 0.026 mM for Km(app) and 7.38 μA for Imax, respectively. Furthermore, the PT-NH2-g-PEG/Lac biofilm was retained 82% of its activity for 12 days indicating excellent recovery as tested with artificial wastewater.
在不断拓展的导电聚合物研究领域中，功能化接枝杂化共聚物尤其在生物医学工程和生物传感应用方面引起了广泛关注。在本研究中，开发了一种基于导电接枝共聚物用于检测酚类化合物的新型生物传感器。通过铃木缩聚反应合成了一种坚固且新颖的材料，即“带有侧链氨基的聚噻吩 - g - 聚乙二醇”（PT - NH₂ - g - PEG）杂化导电聚合物，并通过¹H NMR分析、紫外 - 可见光谱、凝胶渗透色谱（GPC）和荧光光谱对其进行了表征。然后将PT - NH₂ - g - PEG结构用作固定化基质以实现扩展的生物传感功能。在一个典型过程中，漆酶被用作检测酚类化合物的模型酶。通过循环伏安法、电化学阻抗谱、原子力显微镜、荧光显微镜和扫描电子显微镜测量对PT - NH₂ - g - PEG/Lac进行了详细的表面表征。发现最佳pH值和聚合物用量分别为pH 6.5和0.5 mg聚合物，线性范围为0.0025 - 0.05 mM，灵敏度为132.45 μA/mM。PT - NH₂ - g - PEG/Lac的动力学参数分别为Km(app)为0.026 mM，Imax为7.38 μA。此外，PT - NH₂ - g - PEG/Lac生物膜在人工废水中测试时，12天内保留了82%的活性，表明具有出色的恢复能力。
