Tavakoli Javad, Tang Youhong
Medical Device Research Institute, College of Science and Engineering, Flinders University, Adelaide 5042, SA, Australia.
Institute for Nano Scale Science & Technology, College of Science and Engineering, Flinders University, Adelaide 5042, SA, Australia.
Polymers (Basel). 2017 Aug 16;9(8):364. doi: 10.3390/polym9080364.
Biosensors that detect and convert biological reactions to a measurable signal have gained much attention in recent years. Between 1950 and 2017, more than 150,000 papers have been published addressing the applications of biosensors in different industries, but to the best of our knowledge and through careful screening, critical reviews that describe hydrogel based biosensors for biomedical applications are rare. This review discusses the biomedical application of hydrogel based biosensors, based on a search performed through Web of Science Core, PubMed (NLM), and Science Direct online databases for the years 2000⁻2017. In this review, we consider bioreceptors to be immobilized on hydrogel based biosensors, their advantages and disadvantages, and immobilization techniques. We identify the hydrogels that are most favored for this type of biosensor, as well as the predominant transduction strategies. We explain biomedical applications of hydrogel based biosensors including cell metabolite and pathogen detection, tissue engineering, wound healing, and cancer monitoring, and strategies for small biomolecules such as glucose, lactate, urea, and cholesterol detection are identified.
能够检测生物反应并将其转化为可测量信号的生物传感器近年来备受关注。1950年至2017年间，发表了超过15万篇论述生物传感器在不同行业应用的论文，但据我们所知，经过仔细筛选，描述用于生物医学应用的水凝胶基生物传感器的批判性综述却很少见。本综述基于对科学网核心库、PubMed（美国国立医学图书馆）和科学Direct在线数据库在2000 - 2017年期间进行的检索，讨论了水凝胶基生物传感器的生物医学应用。在本综述中，我们考虑固定在水凝胶基生物传感器上的生物受体、它们的优缺点以及固定技术。我们确定了最适合此类生物传感器的水凝胶以及主要的转导策略。我们解释了水凝胶基生物传感器的生物医学应用，包括细胞代谢物和病原体检测、组织工程、伤口愈合以及癌症监测，并确定了检测葡萄糖、乳酸、尿素和胆固醇等小分子的策略。
