Hydrogen production from glucose by Citrobacter freundii.

C. freundii, a member of Enterobacteriaceae was isolated from nearby sewage and characterised. With optimum conditions, its hydrogen production capacity and efficiency was tested in synthetic medium containing glucose as carbon and energy source. C. freundii was grown in a 51 fermentor under batch anaerobic conditions. The total production of gas was 8.91 in the volumetric ratio of 63% H2 and 37% CO2 in 11 hr from 30.8 g glucose. From 1 mole of glucose 1.286 mole of hydrogen was produced (YH2/s). The rate of gas production (rQ) and hydrogen production (rH2) was 0.71 and 0.45 1/hr respectively. The strain appears to be a better one for hydrogen production compared to the earlier Citrobacter spp reported.