Kumar G R, Vatsala T M
Indian J Exp Biol. 1989 Sep;27(9):824-5.
C. freundii, a member of Enterobacteriaceae was isolated from nearby sewage and characterised. With optimum conditions, its hydrogen production capacity and efficiency was tested in synthetic medium containing glucose as carbon and energy source. C. freundii was grown in a 51 fermentor under batch anaerobic conditions. The total production of gas was 8.91 in the volumetric ratio of 63% H2 and 37% CO2 in 11 hr from 30.8 g glucose. From 1 mole of glucose 1.286 mole of hydrogen was produced (YH2/s). The rate of gas production (rQ) and hydrogen production (rH2) was 0.71 and 0.45 1/hr respectively. The strain appears to be a better one for hydrogen production compared to the earlier Citrobacter spp reported.
弗氏柠檬酸杆菌是肠杆菌科的一员，从附近污水中分离并进行了表征。在最佳条件下，以含葡萄糖作为碳源和能源的合成培养基测试了其产氢能力和效率。弗氏柠檬酸杆菌在5L发酵罐中分批厌氧培养。从30.8g葡萄糖在11小时内产生的气体总量为8.91，其中氢气占体积比63%，二氧化碳占37%。从1摩尔葡萄糖产生了1.286摩尔氢气（YH2/s）。产气速率（rQ）和产氢速率（rH2）分别为0.71和0.45L/小时。与先前报道的柠檬酸杆菌属菌株相比，该菌株似乎是一种更优的产氢菌株。
