Gao Feng-Yu, Liu Qun-Ying, Yuan Li, Xuan Shi-Ying
Department of Gastroenterology, Hiser Medical Group of Qingdao, Medical College of Qingdao University, Qingdao, Shandong 266033, P.R. China.
The Maternity Centre, The No. 8 People's Hospital of Qingdao, Qingdao, Shandong 266041, P.R. China.
Mol Med Rep. 2015 Nov;12(5):7005-10. doi: 10.3892/mmr.2015.4253. Epub 2015 Aug 26.
Gastric cancer is one of the most frequent malignancies and a leading cause of cancer-related mortality worldwide. MicroRNAs (miRs), a class of small non‑coding RNAs, have been shown to be critical in tumorigenesis. In the present study, the expression levels of miR‑132 were analyzed in gastric cancer samples using quantitative reverse transcription‑polymerase chain reaction. In addition, the cell viability, proliferation and invasion abilities were determined in two gastric cancer cell lines, NCI‑N87 and MGC80‑3, that were transfected with miR‑132 mimics or antisense oligos. It was found that miR‑132 expression was significantly upregulated in gastric cancer tissues when compared with adjacent non‑cancerous tissues. At the molecular level, the data demonstrated that miR‑132 inhibits the protein levels of retinoblastoma 1 (RB1) by targeting the 3'‑untranslated region. Furthermore, reintroduction of RB1 markedly attenuated the proliferative roles of miR‑132 overexpression. Therefore, the present results indicate that the miR‑132/RB1 regulatory axis may be a potential novel diagnostic and therapeutic target for the treatment of gastric cancer.
胃癌是最常见的恶性肿瘤之一，也是全球癌症相关死亡的主要原因。微小RNA（miR）是一类小的非编码RNA，已被证明在肿瘤发生中起关键作用。在本研究中，使用定量逆转录-聚合酶链反应分析了胃癌样本中miR-132的表达水平。此外，在转染了miR-132模拟物或反义寡核苷酸的两种胃癌细胞系NCI-N87和MGC80-3中测定了细胞活力、增殖和侵袭能力。结果发现，与相邻的非癌组织相比，胃癌组织中miR-132的表达明显上调。在分子水平上，数据表明miR-132通过靶向3'-非翻译区抑制视网膜母细胞瘤1（RB1）的蛋白水平。此外，RB1的重新引入显著减弱了miR-132过表达的增殖作用。因此，本研究结果表明，miR-132/RB1调控轴可能是治疗胃癌的潜在新型诊断和治疗靶点。
