Bagheri Ziari Sedigheh, Naji Tahereh, Hosseinzadeh Sahafi Homayoun
Department of Basic Sciences, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Department of Basic Sciences, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Anim Reprod Sci. 2015 Oct;161:32-9. doi: 10.1016/j.anireprosci.2015.07.009. Epub 2015 Jul 30.
Origanum vulgare is a plant of the mint family that contains phytoestrogens. This study compared the effects of O. vulgare, LHRH-A2, and 17β-estradiol on the ultrastructure of gonadotroph cells and ovarian oogenesis in immature Trichogaster trichopterus. Fish (5.1±0.032cm and 2.1±0.043g, n=150) were randomly divided into four treatment groups (three hormonal treatments and control) and treated intramuscularly at four levels with 17β-estradiol or O. vulgare at 10, 20, 30 and 50mg/kg body weight and with LHRH-A2 at 0.001, 0.002, 0.003, and 0.005mg/kg body weight. There were three control treatments: saline, ethanol and placebo. Fish were kept in 15 tanks, with 10 fish per tank, injected a total of seven doses over 13 days. Gonadosomatic index (GSI) and oocyte diameter were lower (P≤0.05) in the control than in the three hormonal treatments. The highest GSI and oocyte diameter responses were observed in fish treated with 17β-estradiol (2.76±0.23%, 149.8±15.43mm) followed by O. vulgare (1.86±0.18%, 104.3±11.5mm) and LHRH-A2 (1.52±0.12%, 91.75±9.02mm) (P≤0.05). Moreover, there was a significant effect of dose level within all the hormonal treatments (P≤0.05). The effect of treatment on the length and weight was likely GSI. Ovarian tissue results showed faster oogenesis of oocytes in fish treated with O. vulgare, after 17β-estradiol. Ultrastructure of gonadotroph cells demonstrated less stimulation by O. vulgare than by 17β-estradiol and LHRH-A2. This study suggests that compared with the two hormonal treatments, O. vulgare dose-dependently affects ovarian oogenesis and gonadotroph cells.
牛至是一种薄荷科植物，含有植物雌激素。本研究比较了牛至、促性腺激素释放激素类似物A2（LHRH - A2）和17β - 雌二醇对未成熟丝足鲈性腺促性腺细胞超微结构和卵巢卵子发生的影响。将鱼（体长5.1±0.032厘米，体重2.1±0.043克，n = 150）随机分为四个处理组（三种激素处理组和对照组），分别以17β - 雌二醇或牛至10、20、30和50毫克/千克体重以及LHRH - A2 0.001、0.002、0.003和0.005毫克/千克体重的四个水平进行肌肉注射。有三种对照处理：生理盐水、乙醇和安慰剂。将鱼饲养在15个水箱中，每个水箱10条鱼，在13天内共注射七剂。对照组的性腺指数（GSI）和卵母细胞直径低于三种激素处理组（P≤0.05）。在接受17β - 雌二醇处理的鱼中观察到最高的GSI和卵母细胞直径反应（分别为2.76±0.23%，149.8±15.43毫米），其次是牛至（1.86±0.18%，104.3±11.5毫米）和LHRH - A2（1.52±0.12%，91.75±9.02毫米）（P≤0.05）。此外，在所有激素处理中剂量水平都有显著影响（P≤0.05）。处理对体长和体重的影响可能与GSI有关。卵巢组织结果显示，在用牛至处理的鱼中，卵母细胞的卵子发生比用17β - 雌二醇处理的鱼更快。性腺促性腺细胞的超微结构表明，牛至对其的刺激小于17β - 雌二醇和LHRH - A2。本研究表明，与两种激素处理相比，牛至剂量依赖性地影响卵巢卵子发生和性腺促性腺细胞。
