固定和活的果蝇卵室中肌动蛋白细胞骨架动力学的可视化

Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers.

作者信息

Groen Christopher M, Tootle Tina L

机构信息

Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 51 Newton Rd, 1-550 BSB, Iowa City, IA, 52242, USA.

出版信息

Methods Mol Biol. 2015;1328:113-24. doi: 10.1007/978-1-4939-2851-4_8.

DOI:10.1007/978-1-4939-2851-4_8

PMID:26324433

Abstract

Visualization of actin cytoskeletal dynamics is critical for understanding the spatial and temporal regulation of actin remodeling. Drosophila oogenesis provides an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in Drosophila egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.

摘要

肌动蛋白细胞骨架动力学的可视化对于理解肌动蛋白重塑的时空调节至关重要。果蝇卵子发生为可视化肌动蛋白细胞骨架提供了一个出色的模型系统。在这里，我们介绍了通过鬼笔环肽染色在固定样本中以及使用转基因肌动蛋白标记工具在活卵室中对果蝇卵室中的肌动蛋白细胞骨架进行成像的方法。