黑腹果蝇卵巢中核体的荧光原位杂交
Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries.
作者信息
Nizami Zehra F, Liu Ji-Long, Gall Joseph G
机构信息
Department of Embryology, Carnegie Institution for Science, Baltimore, MD, 21218, USA.
出版信息
Methods Mol Biol. 2015;1328:137-49. doi: 10.1007/978-1-4939-2851-4_10.
Abstract
Fluorescent in situ hybridization (FISH) is a technique for determining the cytological localization of RNA or DNA molecules. There are many approaches available for generating in situ hybridization probes and conducting the subsequent hybridization steps. Here, we describe a simple and reliable FISH method to label small RNAs (200-500 nucleotides in length) that are enriched in nuclear bodies in Drosophila melanogaster ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other Drosophila tissues, and to abundant mRNAs such as histone transcripts.
摘要
荧光原位杂交（FISH）是一种用于确定RNA或DNA分子细胞定位的技术。有许多方法可用于生成原位杂交探针并进行后续的杂交步骤。在这里，我们描述了一种简单可靠的FISH方法，用于标记果蝇卵巢中富含核体（如卡哈尔体（CBs）和组蛋白基因座体（HLBs））的小RNA（长度为200 - 500个核苷酸）。该技术也可应用于其他果蝇组织以及丰富的mRNA，如组蛋白转录本。
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