Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
Infect Immun. 2015 Nov;83(11):4373-82. doi: 10.1128/IAI.00466-15. Epub 2015 Aug 31.
Serratia marcescens is a soil- and water-derived bacterium that secretes several host-directed factors and causes hospital infections and community-acquired ocular infections. The putative two-component regulatory system composed of EepR and EepS regulates hemolysis and swarming motility through transcriptional control of the swrW gene and pigment production through control of the pigA-pigN operon. Here, we identify and characterize a role for EepR in regulation of exoenzyme production, stress survival, cytotoxicity to human epithelial cells, and virulence. Genetic analysis supports the model that EepR is in a common pathway with the widely conserved cyclic-AMP receptor protein that regulates protease production. Together, these data introduce a novel regulator of host-pathogen interactions and secreted-protein production.
粘质沙雷氏菌是一种源自土壤和水的细菌，它分泌多种宿主导向因子，导致医院感染和社区获得性眼部感染。由 EepR 和 EepS 组成的假定双组分调节系统通过转录控制 swrW 基因调节溶血和群集运动，并通过控制 pigA-pigN 操纵子调节色素产生。在这里，我们确定并描述了 EepR 在调节外毒素产生、应激生存、对人上皮细胞的细胞毒性和毒力方面的作用。遗传分析支持 EepR 与广泛保守的环 AMP 受体蛋白在共同途径中的模型，该蛋白调节蛋白酶的产生。这些数据共同引入了一种宿主-病原体相互作用和分泌蛋白产生的新型调节剂。
