Nazeih Shaimaa I, Ali Mohamed A M, Halim Alyaa S Abdel, Al-Lawati Hanan, Abbas Hisham A, Al-Zharani Mohammed, Boufahja Fehmi, Alghamdi Mashael A, Hegazy Wael A H, Seleem Noura M
Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
Microorganisms. 2023 Sep 28;11(10):2420. doi: 10.3390/microorganisms11102420.
The problem of antibiotic resistance is a global critical public health concern. In light of the threat of returning to the pre-antibiotic era, new alternative approaches are required such as quorum-sensing (QS) disruption and virulence inhibition, both of which apply no discernible selective pressure on bacteria, therefore mitigating the potential for the development of resistant strains. Bearing in mind the significant role of QS in orchestrating bacterial virulence, disrupting QS becomes essential for effectively diminishing bacterial virulence. This study aimed to assess the potential use of sub-inhibitory concentration (0.25 mg/mL) of glyceryl trinitrate (GTN) to inhibit virulence in and GTN could decrease the expression of virulence genes in both tested bacteria in a significant manner. Histopathological study revealed the ability of GTN to alleviate the congestion in hepatic and renal tissues of infected mice and to reduce bacterial and leukocyte infiltration. This study recommends the use of topical GTN to treat topical infection caused by and in combination with antibiotics.
抗生素耐药性问题是全球关键的公共卫生问题。鉴于有回到抗生素前时代的威胁，需要新的替代方法，如群体感应（QS）干扰和毒力抑制，这两种方法对细菌都不会施加明显的选择压力，因此降低了耐药菌株产生的可能性。考虑到群体感应在协调细菌毒力方面的重要作用，破坏群体感应对于有效降低细菌毒力至关重要。本研究旨在评估亚抑制浓度（0.25毫克/毫升）的硝酸甘油（GTN）抑制[具体细菌名称1]和[具体细菌名称2]毒力的潜在用途，并且GTN能够显著降低两种受试细菌中毒力基因的表达。组织病理学研究表明，GTN能够缓解感染小鼠肝脏和肾脏组织的充血，并减少细菌和白细胞浸润。本研究建议使用局部应用的GTN联合抗生素治疗由[具体细菌名称1]和[具体细菌名称2]引起的局部感染。
