Khayyat Ahdab N, Hegazy Wael A H, Shaldam Moataz A, Mosbah Rasha, Almalki Ahmad J, Ibrahim Tarek S, Khayat Maan T, Khafagy El-Sayed, Soliman Wafaa E, Abbas Hisham A
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
Microorganisms. 2021 May 18;9(5):1083. doi: 10.3390/microorganisms9051083.
is an opportunistic nosocomial pathogen and causes wound and burn infections. It shows high resistance to antibiotics and its pathogenicity is mediated by an arsenal of virulence factors. Another therapeutic option to such infections is targeting quorum sensing (QS), which controls the expression of different virulence factors. Prevention of QS can deprive from its bacterial virulence without applying stress on the bacterial growth and facilitates the eradication of the bacteria by immunity. The objective of the current study is to explore the antimicrobial and antivirulence activities of xylitol against . Xylitol could inhibit the growth of . Sub-inhibitory concentrations of xylitol could inhibit biofilm formation, reduce prodigiosin production, and completely block protease activity. Moreover, xylitol decreased swimming motility, swarming motility and increased the sensitivity to hydrogen peroxide. The expression of , , , , fimC, , and genes that regulate virulence factor production was significantly downregulated by xylitol. In silico study showed that xylitol could bind with the SmaR receptor by hydrophobic interaction and hydrogen bonding, and interfere with the binding of the natural ligand with SmaR receptor. An in vivo mice survival test confirmed the ability of xylitol to protect mice against the virulence of . In conclusion, xylitol is a growth and virulence inhibitor in and can be employed for the treatment of wound and burn infections.
是一种机会性医院病原体，可引起伤口和烧伤感染。它对抗生素表现出高度抗性，其致病性由一系列毒力因子介导。针对此类感染的另一种治疗选择是靶向群体感应（QS），群体感应控制不同毒力因子的表达。预防群体感应可以在不对细菌生长施加压力的情况下剥夺其细菌毒力，并有助于通过免疫清除细菌。本研究的目的是探索木糖醇对……的抗菌和抗毒力活性。木糖醇可以抑制……的生长。亚抑制浓度的木糖醇可以抑制生物膜形成，减少灵菌红素产生，并完全阻断蛋白酶活性。此外，木糖醇降低了游动性、群游性，并增加了对过氧化氢的敏感性。调节毒力因子产生的……、……、……、……、fimC、……和……基因的表达被木糖醇显著下调。计算机模拟研究表明，木糖醇可以通过疏水相互作用和氢键与SmaR受体结合，并干扰天然配体与SmaR受体的结合。体内小鼠存活试验证实了木糖醇保护小鼠免受……毒力影响的能力。总之，木糖醇是……的生长和毒力抑制剂，可用于治疗……伤口和烧伤感染。
