Department of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Melioidosis Research Center, Khon Kaen University, Khon Kaen, Thailand.
Am J Trop Med Hyg. 2014 Mar;90(3):480-5. doi: 10.4269/ajtmh.13-0446. Epub 2014 Jan 20.
Burkholderia pseudomallei causes melioidosis, the third most common cause of death from infectious diseases in northeast Thailand. Four physicochemical factors were set so that their values covered the range of the northeast, which is an endemic area. The soil pH was set at pH 4-10, soil salinity was 0.0-5.0% NaCl, total iron was 50-150 mg/kg soil, and carbon to nitrogen ratio (C/N) was 10:1 to 40:1. The experiments were carried out at 37°C, and soil moisture was maintained for 7 days. The number of viable bacterial cells was counted daily. Soil pH, salinity, Fe, and C/N ratio affected the bacterial growth. The bacterial colony was significantly (P < 0.05) reduced at soil pH > 8, soil salinity > 1% NaCl, and C/N ratio > 40:1. However, the growth of B. pseudomallei was enhanced by increasing the concentrations of iron significantly (P < 0.05). We propose using these findings to control B. pseudomallei in situ.
类鼻疽伯克霍尔德菌引起类鼻疽，是泰国东北部第三大致死传染病。设置了四个物理化学因素，使其值涵盖了流行地区东北部的范围。土壤 pH 值设定为 4-10，土壤盐度为 0.0-5.0%NaCl，总铁含量为 50-150mg/kg 土壤，碳氮比（C/N）为 10:1 至 40:1。实验在 37°C 下进行，土壤水分保持 7 天。每天计数活菌数。土壤 pH 值、盐度、铁和 C/N 比对细菌生长有影响。土壤 pH 值>8、盐度>1%NaCl 和 C/N 比>40:1 时，细菌菌落明显减少（P<0.05）。然而，铁浓度的增加显著促进了类鼻疽伯克霍尔德菌的生长（P<0.05）。我们建议利用这些发现来原位控制类鼻疽伯克霍尔德菌。
