干燥对干血斑（DBS）均匀性的影响。
The effect of drying on the homogeneity of DBS.
作者信息
Lenk Gabriel, Hansson Jonas, Beck Olof, Roxhed Niclas
机构信息
KTH Royal Institute of Technology, Department for Micro & Nanosystems, Osquldas väg 10, 10044 Stockholm, Sweden.
Karolinska University Hospital, Department of Clinical Pharmacology, 14186 Stockholm, Sweden.
出版信息
Bioanalysis. 2015;7(16):1977-85. doi: 10.4155/bio.15.135.
BACKGROUND
Inhomogeneous sample distribution in DBS is a problem for accurate quantitative analysis of DBS, and has often been explained by chromatographic effects.
RESULTS
We present a model describing formation of inhomogeneous DBS during drying of the spot caused by higher evaporation rates of water at the edge as compared with the center. Color intensity analysis shows that the relative humidity and DBS card position affect the homogeneity of DBS.
CONCLUSION
The so-called 'coffee-stain effect' explains the typical distribution pattern of analytes with higher concentrations measured along the edge of DBS as compared with the center. The driving mechanism and potential influencing factors should be considered when addressing the inhomogeneity of DBS in the future.
背景
干血斑（DBS）中样本分布不均匀是准确进行DBS定量分析的一个问题，且常被解释为色谱效应。
结果
我们提出了一个模型，该模型描述了在斑点干燥过程中，由于边缘处水的蒸发速率高于中心，从而导致DBS不均匀形成的过程。颜色强度分析表明，相对湿度和DBS卡位置会影响DBS的均匀性。
结论
所谓的“咖啡斑效应”解释了与中心相比，沿DBS边缘测得的浓度较高的分析物的典型分布模式。未来在解决DBS不均匀性问题时，应考虑其驱动机制和潜在影响因素。
Zotero 插件