Qi Zhihong, Chen Yu, Zhang Lin, Ma Xinming, Wang Fei, Cheng Qian, Du Juan, Hao Yingying, Chi Shuling, Cui Wei
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.
Institute of Basic Medicine Sciences, Chinese Academy of Medical Sciences School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Clin Chim Acta. 2016 Jan 15;452:87-91. doi: 10.1016/j.cca.2015.11.008. Epub 2015 Nov 10.
Reports on biological variation of plasma biochemical indicators are limited. We evaluated biological variations of 13 plasma biochemical indicators.
Plasma samples were collected from 40 healthy individuals over 5days. Intra-individual coefficient of variation (CVI), inter-individual coefficient of variation (CVG), index of individuality (II), reference change value (RCV), and analytical goal parameters were calculated.
Albumin (Alb) showed the lowest CVI (2.50%) and the lowest CVG (5.08%), while C-reactive protein (hsCRP) presented the highest CVI (26.87%) and CVG (61.73%). II values were all less than 1.0. Alb presented the lowest 95% RCV (7.67), while hsCRP showed the highest 95% RCV (74.61). Alb, urea, creatinine (Cr), creatine kinase (CK), and creatine kinase isoenzyme MB (CKMBmass) CVI differed with gender (P<0.05). The CVG of the 13 indicators presented a significant gender difference (P<0.0001). Alb showed the lowest desirable imprecision CV (1.3%), the lowest desirable bias (1.4%), and the lowest desirable total error (3.5%), while hsCRP presented the highest desirable imprecision (13.4%), the highest desirable bias (16.8%), and the highest desirable total error (39.0%).
Our findings add to the database of biological variations of plasma indicators.
关于血浆生化指标生物学变异的报道有限。我们评估了13种血浆生化指标的生物学变异情况。
在5天内从40名健康个体采集血浆样本。计算个体内变异系数（CVI）、个体间变异系数（CVG）、个体性指数（II）、参考变化值（RCV）以及分析目标参数。
白蛋白（Alb）的CVI最低（2.50%），CVG也最低（5.08%），而超敏C反应蛋白（hsCRP）的CVI最高（26.87%），CVG最高（61.73%）。II值均小于1.0。Alb的95%RCV最低（7.67），而hsCRP的95%RCV最高（74.61）。Alb、尿素、肌酐（Cr）、肌酸激酶（CK）和肌酸激酶同工酶MB（CKMBmass）的CVI存在性别差异（P<0.05）。13项指标的CVG存在显著性别差异（P<0.0001）。Alb的理想不精密度CV最低（1.3%），理想偏倚最低（1.4%），理想总误差最低（3.5%），而hsCRP的理想不精密度最高（13.4%），理想偏倚最高（16.8%），理想总误差最高（39.0%）。
我们的研究结果丰富了血浆指标生物学变异的数据库。
