Jach Robert, Dulinska-Litewka Joanna, Laidler Piotr, Szczudrawa Andrzej, Kopera Andrzej, Szczudlik Lukasz, Pawlik Michal, Zajac Krzysztof, Mak Monika, Basta Antoni
Department of Gynecology, Obstetrics and Oncology, Jagiellonian University, Medical College, Krakow, Poland.
Front Biosci (Elite Ed). 2010 Jan 1;2(2):411-23. doi: 10.2741/e101.
Cervical squamous cell carcinoma (SCC) arises from the metaplastic epithelium and develops slowly through dysplastic changes (i.e., cervical intraepithelial neoplasia--CIN) to carcinoma in situ and invasive cancer. There is little data concerning the quantitation of vascular endothelial growth factor (VEGF) and its correlation to the clinical or pathologic characteristics of SCC. This study assessed the expression of VEGF, VEGF-C and their receptor VEGFR-2 in 35 samples of normal cervical tissue, 35--CIN1, 35--CIN2 (25 non-pregnant, 15 pregnant women), 35--CIN3 and 30- SCC. VEGF, VEGF-C and VEGFR-2 were analyzed using RT-PCR, RQ-PCR, immunohistochemical staining and Western blot. VEGF, VEGF-C and VEGFR-2 were not detected in normal cervical epithelium. In CIN and SCC, both forms of VEGF and its receptor were identified, indicating a correlation between the increasing expression and staging of carcinoma. Results show the important role of VEGF in cervical progression and that the switch to the lymphangiogenesis phenotype occurs prior to the stage of invasion likely at CIN2/3.
宫颈鳞状细胞癌（SCC）起源于化生上皮，通过发育异常改变（即宫颈上皮内瘤变——CIN）缓慢发展为原位癌和浸润癌。关于血管内皮生长因子（VEGF）的定量及其与SCC临床或病理特征的相关性的数据很少。本研究评估了35份正常宫颈组织、35份CIN1、35份CIN2（25例非孕妇、15例孕妇）、35份CIN3和30份SCC样本中VEGF、VEGF-C及其受体VEGFR-2的表达。使用逆转录聚合酶链反应（RT-PCR）、相对定量聚合酶链反应（RQ-PCR）、免疫组织化学染色和蛋白质印迹法分析VEGF、VEGF-C和VEGFR-2。在正常宫颈上皮中未检测到VEGF、VEGF-C和VEGFR-2。在CIN和SCC中，两种形式的VEGF及其受体均被鉴定出来，表明表达增加与癌症分期之间存在相关性。结果显示VEGF在宫颈癌进展中起重要作用，并且向淋巴管生成表型的转变可能在CIN2/3阶段浸润之前就已发生。
