Pektaş Abdulkadir, Gemalmaz Hakan, Balkaya Muharrem, Ünsal Cengiz, Yenisey Çiğdem, Kılıçarslan Naciye, Çulhacı Nil
Department of Urology, Faculty of Medicine, Adnan Menderes University, Aydın, Turkey.
Department of Physiology, Faculty of Veterinary, Adnan Menderes University, Aydın, Turkey.
Turk J Urol. 2014 Mar;40(1):46-51. doi: 10.5152/tud.2014.53765.
Renal ischemia-reperfusion injury may occur due to nephron-sparing surgery in patients with a solitary kidney or restricted renal parenchymas. Prophylactic agents do not always achieve their intended effects and may exhibit side effects. The present study was designed to investigate the possible protective effects of lycopene against hypoxia-induced renal damage.
Twelve Wistar rats were used in the study. Female Wistar rats were divided into two groups of six rats each; the first group served as the control, and the second group was treated for two days with oral lycopene (4 mg/kg per day) before surgery. All Wistar rats were subjected to right nephrectomy and abdominal aorta clamping for 45 minutes to induce ischemia, followed by 24 hours of reperfusion. Blood samples were collected from all rats twice before surgery and 24-hours after surgery for analyses of serum urea, creatinine, sodium, and potassium levels. Left nephrectomies were performed following reperfusion. Then histopathological scores were estimated, and malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels in renal tissue samples were measured, and compared between groups.
There were no significant differences between the control, and the lycopene group with respect to postischemic urea, creatinine, or potassium levels. A significant difference between the groups was observed with respect to postischemic sodium levels (p=0.028). Pathological scores were higher in the control group than in the lycopene group (p<0.05). Mean tissue MDA levels in the control group were higher than in the lycopene group (p=0.055). The mean tissue GSH-Px levels were similar in the control, and lycopene groups. The mean GSH levels in the control group were higher than in the lycopene group (p>0.05). The mean tissue SOD levels were similar in the control, and lycopene groups. The mean CAT levels in the control group were higher than in the lycopene group (p>0.05).
Lycopene may have a protective effect on the short-term biochemical and histopathological parameters following renal ischemia/perfusion injury.
在孤立肾或肾实质受限的患者中，肾部分切除术可能会导致肾缺血再灌注损伤。预防性药物并不总能达到预期效果，且可能会出现副作用。本研究旨在探讨番茄红素对缺氧诱导的肾损伤的潜在保护作用。
本研究使用了12只Wistar大鼠。雌性Wistar大鼠被分为两组，每组6只；第一组作为对照组，第二组在手术前连续两天口服番茄红素（每天4mg/kg）。所有Wistar大鼠均接受右肾切除术，并夹闭腹主动脉45分钟以诱导缺血，随后进行24小时的再灌注。在手术前和手术后24小时从所有大鼠采集血样，分析血清尿素、肌酐、钠和钾水平。再灌注后进行左肾切除术。然后评估组织病理学评分，并测量肾组织样本中丙二醛（MDA）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）的水平，并在组间进行比较。
对照组和番茄红素组在缺血后尿素、肌酐或钾水平方面无显著差异。两组在缺血后钠水平方面存在显著差异（p=0.028）。对照组的病理评分高于番茄红素组（p<0.05）。对照组的平均组织MDA水平高于番茄红素组（p=0.055）。对照组和番茄红素组的平均组织GSH-Px水平相似。对照组的平均GSH水平高于番茄红素组（p>0.05）。对照组和番茄红素组的平均组织SOD水平相似。对照组的平均CAT水平高于番茄红素组（p>0.05）。
番茄红素可能对肾缺血/灌注损伤后的短期生化和组织病理学参数具有保护作用。
