Ozkan Tayyar Alp, Karakoyunlu Nihat, Polat Reyhan, Sarıbaş Gülistan Sanem, Şener Nevzat Can, Özdemir Seyda, Peker Kevser, Ünal Dilek, Tuygun Can
Department of Urology, Kocaeli Derince Training and Research Hospital, Saglik Bilimleri University, Ibni Sina Blv 1, 41200, Derince, Kocaeli, Turkey.
Department of Urology, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Saglik Bilimleri University, Ankara, Turkey.
Int Urol Nephrol. 2018 Feb;50(2):217-223. doi: 10.1007/s11255-017-1775-8. Epub 2017 Dec 26.
The ischemia and subsequent reperfusion (IR) which occurs in partial nephrectomy used in the treatment of renal tumors causes loss of parenchyma in the damaged kidney. The aim of this study is to evaluate, both biochemically and histologically, the efficacy of esomeprazole in an ischemia-reperfusion model in rat kidneys.
The rats were randomized into three groups of seven animals each, referred to as the sham, control, and PPI groups. In the sham group, only a laparotomy was performed. In the control group, following laparotomy the left renal artery was dissected and tied for 30-min ischemia. In the PPI group, a vascular route to the tail vein was opened, and 10 mg/kg esomeprazole was administered. After 1 h, the same procedures described for the control group were performed. All the animals were killed 24 h after the procedure. Biochemical analyses were applied for evaluation of oxidant and antioxidant agents in the blood and left kidney of each subject (oxidative markers: malondialdehyde, myeloperoxidase; antioxidant marker: superoxide dismutase). In the histological examination of the kidney tissues stained with hematoxylin-eosin, the TUNEL method was applied in the evaluation of apoptosis.
No statistically significant biochemical difference was determined in the blood and tissue samples. In the histological and apoptosis evaluations, a statistically significant difference was determined between the sham, control, and PPI groups. The median (IQR) values of the TUNEL-positive cells were counted as 1.50 (4) in the sham group, 11.50 (12) in the control group, and 6.00 (9) in the PPI group (p < 0.001).
A protective effect of esomeprazole was confirmed in renal ischemia-reperfusion damage created in an experimental rat model.
在治疗肾肿瘤的部分肾切除术中发生的缺血及随后的再灌注（IR）会导致受损肾脏实质的丧失。本研究旨在通过生化和组织学方法评估埃索美拉唑在大鼠肾脏缺血再灌注模型中的疗效。
将大鼠随机分为三组，每组七只，分别称为假手术组、对照组和质子泵抑制剂（PPI）组。假手术组仅进行剖腹手术。对照组在剖腹手术后解剖并结扎左肾动脉30分钟以造成缺血。PPI组开通尾静脉血管通路，并给予10mg/kg埃索美拉唑。1小时后，进行与对照组相同的操作。所有动物在手术后24小时处死。应用生化分析评估每个受试者血液和左肾中的氧化剂和抗氧化剂（氧化标志物：丙二醛、髓过氧化物酶；抗氧化标志物：超氧化物歧化酶）。在用苏木精-伊红染色的肾脏组织的组织学检查中，采用TUNEL法评估细胞凋亡。
在血液和组织样本中未确定有统计学意义的生化差异。在组织学和细胞凋亡评估中，假手术组、对照组和PPI组之间存在统计学意义的差异。TUNEL阳性细胞的中位数（四分位间距）在假手术组为1.50（4），对照组为11.50（12），PPI组为6.00（9）（p<0.001）。
在实验性大鼠模型中创建的肾缺血再灌注损伤中证实了埃索美拉唑的保护作用。
