Guo Hui, Pang Kunpeng, Wei Yan, Yi Cuihua, Wu Xinyi
Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, China.
Ophthalmic Res. 2015;54(3):128-34. doi: 10.1159/000437209. Epub 2015 Sep 1.
To investigate the role of herpes virus entry mediator (HVEM) in viral entry and inflammatory cytokine production in response to herpes simplex virus (HSV) type 1 challenge in human corneal epithelial cells.
HVEM expression in human corneal epithelial cells was determined by immunofluorescence and flow cytometry. The HSV-1 virus expressing β-galactosidase was used to challenge corneal epithelial cells and viral entry assays were performed to ascertain HSV-1 entry into cells. Levels of cytokines TNF-α, IL-6, IFN-x03B3;, IL-12, and IL-18 and chemokines MIP-1α, MIP-1β and MIP-2 were detected in corneal epithelial cells treated with control or HVEM siRNA in response to HSV-1 challenge.
Human corneal epithelial cells were positive for HVEM expression and showed high susceptibility to HSV-1 entry. Silencing of HVEM did not alter viral entry dramatically. However, levels of the cytokine IFN-x03B3; and chemokines MIP-1α and MIP-1β were measured to be higher in HVEM siRNA-treated cells than control after HSV-1 challenge.
HVEM in human corneal epithelial cells may act to dampen the production of some cytokines and chemokines and thus it may modulate the innate immune response against HSV-1. This may provide a novel mechanism for the pathogenesis of HSV-1 infection in the cornea.
研究疱疹病毒进入介质（HVEM）在人角膜上皮细胞应对1型单纯疱疹病毒（HSV）攻击时病毒进入及炎性细胞因子产生过程中的作用。
通过免疫荧光和流式细胞术测定人角膜上皮细胞中HVEM的表达。使用表达β-半乳糖苷酶的HSV-1病毒攻击角膜上皮细胞，并进行病毒进入试验以确定HSV-1进入细胞的情况。在对照或HVEM siRNA处理的角膜上皮细胞应对HSV-1攻击时，检测细胞因子TNF-α、IL-6、IFN-γ、IL-12和IL-18以及趋化因子MIP-1α、MIP-1β和MIP-2的水平。
人角膜上皮细胞HVEM表达呈阳性，对HSV-1进入表现出高敏感性。HVEM沉默并未显著改变病毒进入。然而，在HSV-1攻击后，经HVEM siRNA处理的细胞中细胞因子IFN-γ以及趋化因子MIP-1α和MIP-1β的水平高于对照组。
人角膜上皮细胞中的HVEM可能起到抑制某些细胞因子和趋化因子产生的作用，因此它可能调节针对HSV-1的固有免疫反应。这可能为角膜中HSV-1感染的发病机制提供一种新机制。
